Background: Recently, it has been revealed that tyrosine kinase inhibitors (TKIs) act through inducing both oxidative and endoplasmic reticulum (ER) stress in chronic myeloid leukemia cells. However, ER stress signaling triggers both apoptotic and survival processes within cells. Nevertheless, mechanisms by which TKIs avoid the pro-survival effects are not clear. The aim of this study was to ev...

conclusions collectively, the v. pumilum extracts showed remarkable cytotoxic activity and merit further investigation as anticancer agents. results the ch2cl2 fraction of v. pumilum showed a potent dose-related inhibitory effect on cell viability. the ch2cl2 fraction showed ic50 values of 8.3 and 12.5 µg/ml in the k562 and hl-60 cell lines, respectively. a sub-g1 peak in the flow cytometry his...

OBJECTIVE Plasma high-density lipoprotein (HDL) level is inversely correlated with the risk of atherosclerosis. However, the cellular mechanism by which HDL exerts antiatherogenic actions is not well understood. In this study, we focus on the lipid components of HDL as mediators of the lipoprotein-induced antiatherogenic actions. METHODS AND RESULTS HDL and sphingosine 1-phosphate (S1P) stimu...

In most habitats, fluctuating environmental conditions create periods of compromised survival for metabolically active organisms. response, various strategies have evolved, including the formation resilient resting cells. We assessed biodiversity lysis-resistant bacteria in three different environments by applying a harsh physicochemical treatment to samples. The bacterial diversity fraction wa...

Background: Various MTT assay methods are proposed to obtain the cell sur- vival parameters.Objective: Determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation.Method: A common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adeno...

  Alzheimer’s disease (AD) is a neurodegenerative disease that was characterized with deposit of beta amyloid (Aβ) aggregate in senile plaque. Oxidative damage to neurons and loss of cholinergic neurons in forebrain region are observed in this disease. Melissa officinalis is a medicinal plant from Lamiaceae family, used traditionally in the treatment of cognitive disorders. It has cholinomimeti...

Porphyra dentata, an edible red macroalgae, is used as a folk medicine in Asia. This study evaluated in vitro and in vivo the protective effect of a sterol fraction from P. dentata against breast cancer linked to tumor-induced myeloid derived-suppressor cells (MDSCs). A sterol fraction containing cholesterol, β -sitosterol, and campesterol was prepared by solvent fractionation of methanol extra...

introduction: electroporation is a practical technique used to transport the molecules across the cell membrane. the utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. it is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the chang...

   The aerial parts of Stachys laxa Boiss. and Buhse.from Siah-bishe in Mazandaran province, Stachys trinervis Aitch. and Hemsl. from Karaj in Alborz province, Stachys subaphylla Rech. F. and Stachys turcomanica Trautv. from Golestan province have been collected in May 2008. Total extracts were obtained through MeOH/H2O (80/20) and then partitioned between CHCl3, EtOAc and MeOH. These fractions...