We report successful non-adherent spheroid culture of cancer cells on hydrophobic surfaces, formed in polydimethylsiloxane (PDMS), and its extension to high-throughput culture within an integrated microfluidic device. The surface hydrophobicity was characterized by observing the contact angle of deionized water droplets. Various cell types were grown on the hydrophobic designs (circular, triang...

Objective(s): This study was designed to determine the relationship of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli isolates in multispecies biofilms and their individual phenotypic characters in biofilm consortia. Materials and Methods:  The subject isolates were recovered from different food samples and identified on the basis of growth on differential and selective med...

The porous gas diffusion layers (GDLs) are key components in hydrogen fuel cells. During their operation the cells produce water at the cathode, and to avoid flooding, the water has to be removed out of the cells. How to manage the water is therefore an important issue in fuel cell design. In this paper we investigated water flow in the GDLs using a combination of the lattice Boltzmann method a...

Staphylococcus aureus is found in a wide variety of habitats, including human skin, where many strains are commensals that may be clinically significant or contaminants of food. To determine the physiological characteristics of resistant strain of Staphylococcus aureus against pediocin, a class IIa bacteriocin, a resistant strain was compared with wild type in order to investigate the contribut...

The importance of E. coli as an indicator organism in fresh water has led to numerous studies focusing on cell properties and transport behavior. However, previous studies have been unable to assess if differences in E. coli cell surface properties and genomic variation are associated with different environmental habitats. In this study, we investigated the variation in characteristics of E. co...

BACKGROUND Mucosal surface hydrophobicity is a key factor of the gastric acid defence barrier. In the colon, surface hydrophobicity is high but its biological function remains unexplored. AIMS To investigate the functional changes of the barrier due to removal of the surface active phospholipid layer by a detergent, or to reinforcement of the surface active phospholipid by local application o...

Urinary Tract infections are one of the most common bacterial infections in human. On the present study urine sample of suspected UTI patients were included. Total 23 samples were found to be positive for bacterial infection in which predominant species was Escherichia coli 13 (56.52%) and 10 (43.48%) were found to be other bacterial species i.e. Proteus spp. 4 (17.3%), Citrobacter spp. 3 (13%)...

OBJECTIVE To determine whether several topical compounds and other chemical entities are able to diminish the surface hydrophobicity of yeast cells. METHOD Hydrophobicity of yeast cells was determined by binding styrene microspheres to the surface of untreated yeast or yeast pre-incubated with various substances with potential for cell surface modification. The degree of microsphere adherence...