Background: Using natural compounds with low toxicity on normal cells and high efficacy on malignant cells is highly appreciated for treatment of colorectal cancer (CRC). In the present study, the effect of fish-oil derived eicosapentaenoic acid (EPA) on the cell number, cell proliferation rate and caspase-3 enzyme activity in LS174T human colorectal cancer cell line was investigated. Methods:...

mtt assay is designed for spectrophotometric quantification of cell growth and viability without using the radiactive isotopes. the aim of this study is com¬parison of ltt and mtt as sensitive poliferation assays in immunodeficient pa¬tients. 20 immunodeficient and 20 healthy subjects were selected in this study. all of them had normal lymphocytes count, normal cd3 and negative dth reac¬tion to...

Background: Progesterone is an endogenous immunomodulator that suppresses T cell activation during pregnancy. The stimulation of membrane progesterone receptors (mPRs) would seem to be the cause of rapid non-genomic responses in human peripheral T cells, such as an elevation of intracellular calcium ([Ca2+] i) and decreased intracellular pH (pHi). Mifepristoneimmune cells compared with progeste...

objective(s):eukaryotic translation initiation factor 4e (eif4e) is overexpressed in cervical cancer (cc). however, the molecular mechanisms are unclear. this study aimed to investigate the molecular mechanism of eif4e gene overexpression in cc. materials andmethods:the human papillomavirus (hpv) type 18 e7 and eif4e mrnas were measured following knock down or overexpression of e7 gene by rt-pc...

Dates fruit has been used as staple food in the Middle East for thousands of years and various types of dates are found worldwide. Dates and their constituents show various roles in diseases prevention and treatment through anti-oxidant, anti-inflammatory, anti-bacterial activity. In the present study we investigated the activity of aqueous and n-hexane extracts of Phoenix dactylifera L. fruit ...

background: a growing body of preclinical data indicates that statins may possess antineoplastic properties however, some studies have raised the possibility that statins may also have carcinogenic potential. methods: an air pouch model was used for angiogenesis. single or multiple applications of croton oil on the back of swiss albino mice with or without initiation by dimethylbenz(a)antherace...

we aimed to develop a cost-effective and robust method to predict drug resistance in individualpatients. representative tissue fragments were obtained from tumors removed from femalepatients, aged 24-74 years old. the tumor tissue was taken by a histopathology’s or a surgeonunder sterile conditions. cells obtained by enzymatic dissociation from tumor after surgery, werecultured as a monolayer f...

Chemical investigation of Garcinia eugenifolia and Calophyllum enervosum yielded six compounds. One of these was found to be a novel compound identified as enervosanone. Five known compounds cambogin, epicatechin, osajaxanthone, rubraxanthone and isocowanol, were also isolated. These compounds were tested for their bioactivity as antimicrobial, antioxidant and cytotoxicagents. Antimicrobial...

introduction: the native inability of nervous system to regenerate, encourage researchers to consider neural tissue engineering as a potential treatment for spinal cord injuries. considering the suitable characteristics of induced pluripotent stem cells (ipscs) for tissue regeneration applications, in this study we investigated the adhesion, viability and proliferation of neural progenitors (de...

spermatogonia are the male germ line stem cells whose life long expansion is needed for permanent production of spermatozoa. the present study was designed to examine the effect of hcg treatment on germ cell proliferation following stem cell transplantation in mice. spermatogonial stem cells were isolated from neonatal mice testes and characterized by alkaline phosphatase, immunoreactivity and ...