His-tags are widely used for the purification of recombinant proteins. High-cost carriers functionalized with nickel ions commonly required selective immobilization His-tagged enzymes. In this study, varying lengths were fused to N-terminus D-amino acid oxidase (DAO) from Trigonopsis variabilis. The attachment a His6 tag significantly improved solubility DAO expressed in Escherichia coli. By mo...

Several cultures of gram-negative and gram-positive bacteria were successfully immobilized with titanous hydroxide. The immobilization efficiency for the microorganisms investigated in saline and broth media ranged from 80.2 to 99.9%. The immobilization of salmonellae was effective over a wide pH range. The presence of buffers, particularly phosphate buffer, drastically reduced the immobilizati...

Muscular dystrophies are often caused by genetic alterations in the dystrophin-dystroglycan complex or its extracellular ligands. These structures are associated with the cell membrane and provide mechanical links between the cytoskeleton and the matrix. Mechanical stress is considered a pathological mechanism and muscle immobilization has been shown to be beneficial in some mouse models of mus...

Recent outbreaks of food borne illnesses continue to support the need for rapid and sensitive methods for detection of foodborne pathogens. A method for detecting Listeria monocytogenes in food samples was developed using an automated fiber-optic-based immunosensor, RAPTOR. Detection of L. monocytogenes in phosphate buffered saline (PBS) was performed to evaluate both static and flow through an...

We recently reported that immobilization stress increased colonic motility, mucin, and prostaglandin E2(PGE2) release and mucosal mast cell degranulation in rat colon [ Proc. Natl. Acad. Sci. USA 93: 12611-12615, 1996; Am. J. Physiol. 271 ( Gastrointest. Liver Physiol. 34): G884-G892, 1996]. To directly assess the contribution of mast cells, we compared colonic responses to stress in mast cell-...

Various supports and immobilization/encapsulation techniques have been proposed and tested for application in functional food production. In the present review, the use of probiotic microorganisms for the production of novel foods is discussed, while the benefits and criteria of using probiotic cultures are analyzed. Subsequently, immobilization/encapsulation applications in the food industry a...

The serotoninergic system and the 5-HT1A receptors have been involved in the brain response to acute stress. The aim of our study was evaluate the role of the 5-HT1A receptors in serotoninergic cells of rostral and caudal raphe nuclei under acute immobilization in rats. Double immunocytochemical staining of 5-hydroxy-tryptamine and c-Fos protein and stereology techniques were used to study the ...

The aim of our study was to evaluate the effect of acute stress and the 5-HT(1A) receptor involvement in both, the hippocampus noradrenaline (NA) tissue levels and the c-Fos immunoreactivity (c-Fos-IR) in the catecholaminergic neurons of the locus coeruleus (LC). Double immunocytochemical staining of tyrosine hydroxilase (TH) and c-Fos protein combined with stereological techniques were used to...

Cell-based biosensors have become a research hotspot in biosensors and bioelectronics fields. The main feature of cell-based biosensors is the immobilization of living cell on the surface of transducers. Different types of polymers which are used as scaffolds for cell growth should be biocompatible and have reactive functional groups for further attachment of biomolecules. In this work, the cel...