The low number of CD4+ CD25+ regulatory T cells (Tregs), their anergic phenotype, and diverse antigen specificity present major challenges to harnessing this potent tolerogenic population to treat autoimmunity and transplant rejection. In this study, we describe a robust method to expand antigen-specific Tregs from autoimmune-prone nonobese diabetic mice. Purified CD4+ CD25+ Tregs were expanded...

Treatment with monoclonal antibodies (mAbs) specific for CD25 (anti-CD25 mAb) has been shown to suppress growth of a variety of different tumours in mice. These studies did not however determine whether or not antiCD25 mAbs facilitate tumour rejection by depletion of regulatory T cells or by binding to tumour-specific effector cells. Using a murine model of melanoma we have found that treatment...

Abstract ROS are small short-lived oxygen-containing molecules which highly chemically reactive and involved in the regulation of biological processes like T cell activation proliferation. The tumor microenvironment (TME) is a metabolically hostile environment with deficiency nutrients glucose glutamine, accumulation tumor-driven metabolic waste, extreme hypoxia. Hypoxia results impaired mitoch...

Purpose Beyond the limited scope of non-specific polyclonal regulatory T cell (Treg)-based immunotherapy, which depends largely on serendipity, the present study explored a target Treg subset appropriate for the delivery of a novel epitope spreader Pep19 antigen as part of a sophisticated form of immunotherapy with defined antigen specificity that induces immune tolerance. Methods Human polyc...

In spite of an initially proposed role as a costimulatory molecule for CD69, in vivo studies showed it as a regulator of immune responses and lymphocyte egress. We found constitutive CD69 expression by T cell subsets and pDC. We examined a possible effect of CD69 on T cell proliferation using transfer models and in vitro assays. In mice locally expressing or receiving antigen, anti-CD692.2 trea...

Chronic activity of hepatitis B is thought to involve aberrant immune tolerance of unknown mechanism. In this study, we examined the role of CD4(+)CD25(+)Foxp3(+) regulatory T cells in disease activity and viral clearance in hepatitis B. Patients with chronic active hepatitis B (CAH) and asymptomatic HBV carriers (AsC) exhibited a significantly high frequency of CD4(+)CD25(+)Foxp3(+) T cells as...

BACKGROUND AND PURPOSE The aim of this study was to investigate the frequency of CD4(+)CD25(+) regulatory T cells and their phenotypic expression in peripheral blood of children with active and non-active juvenile idiopathic arthritis (JIA) and healthy controls, to determine if their frequency or phenotypic expression is involved in the immunoregulation of this disease. METHODS From October 2...

Naturally occurring CD4+CD25+ regulatory T cells (Tregs) are essential for the suppression of autoimmunity and can control the immune-mediated pathology during the early phase of sepsis. Our previous data showed that silencing interleukin-37 (IL-37) in human CD4+CD25+ Tregs obviously reduced the suppressive activity of CD4+CD25+ Tregs. Here, we found that rhIL-37 stimulation markedly enhanced t...

stem cell transplantation in children following reduced intensity conditioning: potential predictive value of NK cell chimaerism for late graft rejection. Leukemia. 2003;17:1934-1942. 7. Seidel MG, Ernst U, Printz D, et al. Expression of the putatively regulatory T-cell marker FOXP3 by CD4( )CD25 T cells after pediatric hematopoietic stem cell transplantation. Haematologica. 2006;91:566-569. 8....