Secretory granule (SG) maturation has been proposed to involve formation of clathrin-coated vesicles (CCVs) from immature SGs (ISGs). We tested the effect of inhibiting CCV budding by using the clathrin adaptor GGA (Golgi-associated, gamma-ear-containing, ADP-ribosylation factor-binding protein) on SG maturation in neuroendocrine cells. Overexpression of a truncated, GFP-tagged GGA, VHS (Vps27,...

In diverse species, actin assembly facilitates clathrin-coated vesicle (CCV) formation during endocytosis. This role might be an adaptation specific to the unique environment at the cell cortex, or it might be fundamental, facilitating CCV formation on different membranes. Proteins of the Sla2p/Hip1R family bind to actin and clathrin at endocytic sites in yeast and mammals. We hypothesized that...

Many soluble proteins transit through the trans-Golgi network (TGN) and the prevacuolar compartment (PVC) en route to the vacuole, but our mechanistic understanding of this vectorial trafficking step in plants is limited. In particular, it is unknown whether clathrin-coated vesicles (CCVs) participate in this transport step. Through a screen for modified transport to the vacuole (mtv) mutants t...

A comprehensive investigation on diesel pilot spray ignited methane-hydrogen (CH 4 –H 2 ) combustion, tri-fuel combustion (TF), is performed in a single-cylinder compression ignition (CI) engine. The experiments provide detailed analysis of the effect H concentration (based mole fraction, M H2 and charge-air temperature (T air behavior, stability, cycle-to-cycle (CCV) engine performance. result...

Viability and fertility components of selection associated with linkage blocks marked by four electrophoretically detectable loci were estimated in an experimental population of barley [Composite Cross V (CCV)]. The intensity of selection affecting the distribution of pollen types in the outcross pool was also estimated and comparisons were made between the selective values of genes in the pool...

The modulation of spinal cord excitability during rhythmic limb movement reflects the neuronal coordination underlying actions of the arms and legs. Integration of network activity in the spinal cord can be assessed by reflex variability between the limbs, an approach so far very little studied. The present work addresses this question by eliciting Hoffmann (H-) reflexes in both limbs to assess...

Cycle-to-cycle variations (CCV) in spark-ignited (SI) engines impose performance limitations and the extreme limit can lead to very strong, potentially damaging cycles. Thus, CCV force sub-optimal engine operating conditions. A deeper understanding of is key enabling control strategies, improving design reducing negative impact on operation. This paper presents a new simulation strategy which a...

Esta pesquisa tem como objetivo mapear o percurso percorrido pela criança na produção das líquidas /l, ɾ/ em ataques simples e complexos Português Brasileiro, delineando a interação entre aquisição segmental silábica durante desenvolvimento fonológico. Um estudo experimental comparando de 71 crianças 2;0 5;11 anos foi conduzido para responder três questões: i) CCV ocorre somente após CV, ou pod...