Feces collected from 541 milking cows on two dairy farms each in Vermont, New York, Pennsylvania, Maryland, Virginia, North Carolina, and Florida were examined for the presence of Cryptosporidium oocysts. Oocysts were concentrated from 15 g of feces from each cow and DNA was extracted. A two-step nested PCR protocol was used to amplify an 830 base pair fragment of the SSUrRNA gene. PCR-positive...

Shiga toxin-producing Escherichia coli (STEC) strains possessing genes for enterohemolysin (ehxA) and/or intimin (eae), referred to here as complex STEC (cSTEC), are more commonly recovered from the feces of humans with hemolytic uremic syndrome and hemorrhagic colitis than STEC strains that do not possess these accessory virulence genes. Ruminants, particularly cattle and sheep, are recognized...

The current study represents a comprehensive investigation of the occurrence and fates trenbolone acetate (TBA) metabolites 17?-trenbolone (17?-TBOH), 17?-TBOH, trendione (TBO); melengesterol (MGA); less commonly studied ?-andrenergic agonist ractopamine (RAC) in two 8 month cattle feeding trials simulated rainfall runoff experiments. Cattle were administered TBA, MGA, or RAC, their residues me...

Objective: Salmonellosis is a bacterial disease with a rising prevalence in the cattle industry. It is most common in dairy calves one to ten weeks of age. Salmonellosis has a serious economic impact on the cattle industry worldwide. Methods: In May 2013, a three months old male Holstein calf with the history of lateral recumbency was referred to veterinary hospital with severe depression, cach...

Objective: Salmonellosis is a bacterial disease with a rising prevalence in the cattle industry. It is most common in dairy calves one to ten weeks of age. Salmonellosis has a serious economic impact on the cattle industry worldwide. Methods: In May 2013, a three months old male Holstein calf with the history of lateral recumbency was referred to veterinary hospital with severe depression, cach...

The natural abundance of 15N (expressed as delta 15N per mil relative to air N2) in the tissues and the change of 15N abundance during the digestion processess in cattle, pig, and goat were investigated. The 15N abundances of cattle tissues differed by about 3.5% with high values in heart, urinary bladder and diaphragm. The ages of cattle did not affect on the 15N abundances of liver and kidney...

Salmonella is a significant food safety concern in commercial beef production, and some contamination thought to occur by inclusion of -infected peripheral lymph nodes (LN) ground through fecal contamination. Surveillance processing plants assists packers risk management understanding seasonal trends risks associated with different cattle types. Approximately 25 samples 20 LN were collected fro...

The isolation and quantification of non-O157 Shiga toxin-producing Escherichia coli (STEC) from cattle feces are challenging. The primary objective of this study was to evaluate the performance of selected agar media in an attempt to identify an optimal medium for the detection and quantification of non-O157 STEC in cattle feces. Comparison studies were performed using CHROMagar STEC, Possé dif...

Objective:To give updated information regarding the occurrence of methicillin-susceptible and methicillin-resistant S. aureus isolated from dairy cattle buffalos in a linked study population. Design: Descriptive study. Samples:The comprised 360 samples (240 animal origin 120 humans). Three different types (including teat swabs, milk feces, 80 each) were collected animals addition (nasal hand ...