Ricinoleic acid is a kind of unsaturated fatty acids in oil castor bean (Ricinus communis) seeds with wide application value. However, there little transcriptomic information on genes related to ricinoleic biosynthesis beans. To better understand the regulation mechanism biosynthesis, immature at three developmental stages (S1, S2, and S3 corresponding 15, 30, 45 d after pollination) were colle...

Two isoenzymes each of phosphoglucomutase, hexose phosphate isomerase, aldolase, fructose diphosphatase, phosphofructokinase, and 6-phosphogluconate dehydrogenase have been separated by DEAE-cellulose column chromatography of extracts from endosperm of germinating castor beans (Ricinus communis cv. Hale). One of each of the enzymes is localized in the cytosol and the other is confined to plasti...

Recent years have seen an increase in the forensic interest associated with the poison ricin, which is extracted from the seeds of the Ricinus communis plant. Both light element (C, N, O, and H) and strontium (Sr) isotope ratios have previously been used to associate organic material with geographic regions of origin. We present a Bayesian integration methodology that can more accurately predic...

1. Lipid extracts were obtained from castor-bean endosperm tissue at various times during germination and, after purification, the total lipid content was determined. Quantitative measurements of the triglyceride and phospholipid content together with the fatty acid composition were made. 2. The total lipid content of the endosperm rapidly decreased during germination; after 10 days less than 2...

Oleate Delta(12)-hydroxylase activity was measured in extracts of developing castor bean seeds. Most of the hydroxylase activity is associated with microsomes. However, when microsomes are washed, the activity is completely lost. Some (50%) of the activity can be restored by addition of the 100,000g supernatant to the washed microsomes. Supernatant extracts (100,000g) of developing safflower se...

Ribulose 1,5-diphosphate (RuDP) carboxylase has been partially purified from dark-grown nonphotosynthetic endosperms of germinating castor beans (Ricinus communis var. Hale). The Km values for RuDP, HCO(3) (-), and Mg(2+) are 0.51, 33, and 1.78 mm, respectively. The pH optimum for the carboxylation reaction is pH 7.5. Germination is required for the development of the carboxylase in the endospe...

Peroxisomes have been identified on the basis of morphological criteria in a variety of species (Hruban and Rechcigl, 1969). Biochemical characterization, however, has been limited to rat liver (deDuve & Baudhuin, 1966; Leighton, et al., 1968), rat kidney (Allen et al., 1965; deDuve & Baudhuin, 1966), Tetrahymena pyriformis (Baudhuin et al., 1965b; Miiller et al., 1968), Acanrhoamoeba (Miiller ...