We report the identification and characterization of two distinct GnRH receptor (GnRH-R) subtypes, designated GnRH-R1 and GnRH-R2, in a model teleost, the medaka Oryzias latipes. These seven-transmembrane receptors of the medaka contain a cytoplasmic C-terminal tail, which has been found in all other nonmammalian GnRH-Rs cloned to date. The GnRH-R1 gene is composed of three exons separated by t...

Given an undirected graph with edge costs and edge demands, the Capacitated Arc Routing problem (CARP) asks for minimum-cost routes for equal-capacity vehicles so as to satisfy all demands. Constant-factor polynomial-time approximation algorithms were proposed for CARP with triangle inequality, while CARP was claimed to be NP-hard to approximate within any constant factor in general. Correcting...

OBJECTIVE Good biomarkers are important to guide decisions in the clinical management of rheumatoid arthritis (RA). RA patients harbor antibodies directed against carbamylated proteins which may predict joint damage. This study investigated whether antibodies against carbamylated proteins (anti-CarP) may serve as surrogate prognostic markers. MATERIAL AND METHODS Fifty-three patients with a d...

This paper highlights the present status and genetic improvement strategies for endemic and exotic carp species of Bangladesh, including future plans. In this country, both major and minor endemic and exotic (introduced) carps are the key species for aquaculture, particularly for polyculture. There are at least 13 endemic species of carps: among them 4 major carps (catla, rohu, mrigal and calba...

CARP-CG is a conjugate gradient (CG) acceleration of CARP, which was introduced by Gordon and Gordon as a robust block-parallel scheme for sparse linear systems. CARP performs Kaczmarz (KACZ) row projections within the blocks, and the results from the separate blocks are merged by averaging, for each component, its updated values from the different blocks. The averaging operations are equivalen...

The common carp is one of the most important cultivated species in the world of freshwater aquaculture. The cultivation of this species is particularly productive due to its high skeletal muscle mass; however, the molecular mechanisms of skeletal muscle development in the common carp remain unknown. It has been shown that a class of non-coding ∼22 nucleotide RNAs called microRNAs (miRNAs) play ...

CARP, a cardiac doxorubicin (adriamycin)-responsive protein, has been identified as a nuclear protein whose expression is downregulated in response to doxorubicin. In the present study, we tested the hypothesis that CARP serves as a reliable genetic marker of cardiac hypertrophy in vivo and in vitro. CARP expression was markedly increased in 3 distinct models of cardiac hypertrophy in rats: con...

The majority of human endometrial and ovarian cancers express receptors for GnRH type I (GnRH-I). Their proliferation is time- and dose-dependently reduced by GnRH-I and its analogs. GnRH-I analogs activate a phosphotyrosine-phosphatase (PTP) and inhibit EGF-induced mitogenic signal transduction. Recently we found that GnRH type II (GnRH-II) and its agonist [D-Lys6]GnRH-II also have antiprolife...

The recently cloned lamprey GnRH receptor was shown to have several unique features, including the longest intracellular C-terminal tail (120 amino acids (aa)) of any previously described GnRH receptor. In the current study, a series of experiments were performed examining cAMP responses, binding kinetics, whole cell competitive binding assays and internalization studies of the lamprey GnRH rec...

Gonadotrophin-releasing hormone (GnRH) is an important factor in the regulation of the synthesis and secretion of gonadotrophins from the pituitary gland. An isoform of this decapeptide, GnRH-II, with an amino acid sequence 70% homologous to GnRH-I, has been recently described. Since the physiological effects of GnRH-II are not yet known, we undertook the present study to see whether GnRH-II co...