نتایج جستجو برای: canavan

تعداد نتایج: 211  

2011
Nadine Mersmann Dmitri Tkachev Ruth Jelinek Philipp Thomas Röth Wiebke Möbius Torben Ruhwedel Sabine Rühle Wolfgang Weber-Fahr Alexander Sartorius Matthias Klugmann

Canavan Disease (CD) is a recessive leukodystrophy caused by loss of function mutations in the gene encoding aspartoacylase (ASPA), an oligodendrocyte-enriched enzyme that hydrolyses N-acetylaspartate (NAA) to acetate and aspartate. The neurological phenotypes of different rodent models of CD vary considerably. Here we report on a novel targeted aspa mouse mutant expressing the bacterial β-Gala...

Journal: :Journal of visualized experiments : JoVE 2016
Sarah Jolly Alexander Fudge Nigel Pringle William D Richardson Huiliang Li

Detection of gene expression in different types of brain cells e.g., neurons, astrocytes, oligodendrocytes, oligodendrocyte precursors and microglia, can be hampered by the lack of specific primary or secondary antibodies for immunostaining. Here we describe a protocol to detect the expression of three different genes in the same brain section using double fluorescence in situ hybridization wit...

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