Cover figure: Calcofluor staining illustrating the random budding pattern of ∆twf1/ ∆twf1yeast cells.

Malassezia spp. are lipid-dependent yeasts that have been related to skin mycobiota and dermatological systemic diseases. Study of lipid droplets (LDs) is relevant elucidate the unknown role these organelles in gain a broader overview metabolism Malassezia. Here, we standardized two protocols for analysis LDs M. pachydermatis globosa. The first describes co-staining confocal laser-scanning fluo...

Abstract Rice false smut, caused by Ustilaginoidea virens , is one of the most destructive diseases in major world rice-producing regions. Basic leucine zipper (bZIP) proteins, which belong to an evolutionarily conserved transcription factor family and play critical roles various biological processes eukaryotes, have been previously identified U. ; however, their functions still need be further...

Biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. Biofilms of Thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (Con A or PNA), calcofluor or periodic acid (polysaccharides target), DNase (DNA target), and lipase (triglycerides target). Neither proteas...

Relevance. A number of methods are used to diagnose dermatophytosis in the practice doctors, but their effectiveness varies. The aim study was compare different clinical and laboratory diagnosis dermatophytosis. Methods. 54 hair samples taken from small pets were examined for presence dermatophytes. Diagnostics carried out using a Wood lamp, direct microscopy, fluorescent microscopy with calcof...

Expression of the Saccharomyces cerevisiae DPM1 gene (coding for dolichylphosphate mannose synthase) in Trichoderma reesei (Hypocrea jecorina) increases the intensity of protein glycosylation and secretion and causes ultrastructural changes in the fungal cell wall. In the present work, we undertook further biochemical and morphological characterization of the DPM1-expressing T. reesei strains. ...