Mutation of the sesquiterpene synthase Cop2 was conducted with a high-throughput screen for the cyclization activity using a non-natural substrate. A mutant of Cop2 was identified that contained three amino acid substitutions. This mutant, 17H2, converted the natural substrate FPP into germacrene D-4-ol with 77% selectivity. This selectivity is in contrast to that of the parent enzyme in which ...

The chemical composition and antimicrobial activity of essential oils obtained from leaves of two Myrtaceae species-Eugenia uniflora L. and Plinia trunciflora (O. Berg) Kausel-were determined. Analysis by GC/MS as well as determination of Kovatz indexes indicated atractylone (26.78%) and curzerene (17.96%) as major constituents of E. uniflora oil and α-cadinol (19.15%), apiole (11.15%) and cube...

The essential oil composition of six species of wild Cinnamomum found in Borneo was investigated. The oils were obtained from bark by hydrodistillation and the volatile chemical profile was obtained via Gas Chromatography-Mass Spectrometry (GCMS). A total of 65 volatile constituents were identified, where the essential oils of the studied specimens contained high contents of oxygenated monoterp...

Background:Ocimum forskolei and Teucrium yemense (Lamiaceae) are used in traditional medicine in Yemen. Methods: The chemical composition, antimicrobial, antioxidant and cytotoxic activities of the essential oils isolated from the leaves of Ocimum forskolei Benth. (EOOF) and two different populations of Teucrium yemense Deflers., one collected from Dhamar province (EOTY-d), and another collecte...

Tinh dầu từ lá và cành của loài Bách tán Đài Loan (Taiwania crypomerioides) thu mẫu tại Văn Bàn, Lào Cai, được chưng cất bằng phương pháp lôi cuốn hơi nước hồi lưu. Hàm lượng tinh đạt 0,14 % 0,18% (theo nguyên liệu khô không khí). có màu vàng nhạt, nhẹ hơn nước. Bằng sắc ký khí ghép khối phổ (GC/MS), 47 hợp chất đã xác định, những thành phần chính gồm: α-pinene (36,84%), limonene (27,04%), α-te...

Combined withering and shaking processes are key steps in the formation of characteristic floral fruity aromas black teas. However, effect sequencing on volatile compounds aroma tea has not been evaluated to date. This study used headspace solid-phase microextraction gas chromatography-tandem mass spectrometry detect teas after withering-shaking shaking-withering processing. Five main different...

Myrcia tomentosa, popularly known as “guava-brava”, belongs to the Myrtaceae family, widely used in folk medicine treat various diseases. There already are studies proving effectiveness of several its components. However, there few specific articles on biological potential M. tomentosa compounds. The aim this study was evaluate antineoplastic activity from tomentosa. For these, we performed sil...

Ocimum basilicum essential oil (EO) was evaluated for its biological effects on M. domestica. Characterization of O. EO revealed the presence methyl chavicol (70.93%), linalool (9.34%), epi-?-cadinol (3.69 %), eugenol (2.48%), ?-cadinene (1.67%), 1,8-cineole (1.30%) and (E)-?-ocimene (1.11%). The basil constituents elicited a neuronal response in female adults Adult flies showed reduced prefere...