Phospho-MurNAc-pentapeptide translocase activity in the membrane of M. luteus was lost upon addition of the detergent, Triton X-100, but could be restored by addition of lipid fractions to the assay. By assay in the presence of lipid, the activity of the Triton-solubilized enzyme could be measured. The synthesis of C(55)-isoprenyl-P-P-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide required C(...

The inhibition by bacitracin of the enzymatic dephosphorylation of C(55)-isoprenyl pyrophosphate is abolished by the addition of chelating agents. If, however, the chelating agent is added after a preincubation of bacitracin with a divalent cation and the lipid substrate, then its addition has little effect, indicating that bacitracin, metal ion, and C(55)-isoprenyl pyrophosphate form a complex...

Acidithiobacillus ferrooxidans (A. ferrooxidans, formerly Thiobacillus ferrooxidans) is the Gram-negative, acidophilic, chemolithoautotrophic bacterium that obtains its energy from the oxidation of ferrous iron, elemental sulphur, or partially oxidized sulphur compounds (Leduc and Ferroni, 1994; Rohwerder et al., 2003), even hydrogen or formate in anoxic conditions (Drobner et al., 1990; Pronk ...

A combination of acetate oxidation and acetoclastic methanogenesis has been previously identified to enable high-rate methanogenesis at high temperatures (55 to 65°C), but this capability had not been linked to any key organisms. This study combined RNA-stable isotope probing on 13C-labelled acetate and 16S amplicon sequencing to identify the active micro-organisms involved in high-rate methano...

This paper reviews the accelerated thermal cycling test methods that are currently used by industry to characterize the interconnect reliability of commercial-off-the-shelf (COTS) ball grid array (BGA) and chip scale package (CSP) assemblies. Acceleration induced failure mechanisms varied from conventional surface mount (SM) failures for CSPs. Examples of unrealistic life projections for other ...

The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat. Two PLFAs, denoted T1 and T2, were detected only in the self-heated peat. In...

Previous studies have revealed an increased fractional anisotropy and greater thickness in the anterior parts of the corpus callosum in meditation practitioners compared with control subjects. Altered callosal features may be associated with an altered inter-hemispheric integration and the degree of brain asymmetry may also be shifted in meditation practitioners. Therefore, we investigated diff...

This chapter surveys some recent work on the application of techniques from computational geometry to geometric problems arising in manufacturing processes such as layered manufacturing, mold design, and numerically controlled machining. Within each topic, we discuss problems that have benefited from the application of geometric techniques, and mention several other problems where such techniqu...

The objectives of this study were to determine the selection potential (genetic variation) of sow efficiency traits (prolificacy, longevity), to understand the co-responses (genetic correlations) among sow efficiency traits and other economically important traits (performance, carcass quality, and meat quality), and determine the feasibility of developing breeding value estimates for sow effici...