Introduction: Busulfan as a chemotherapeutic agent causes testicular germinal epithelium depletion and cytotoxicity in germ cells. The aim of this study was to assess antioxidant status, reactive oxygen species (ROS) generation and apoptosis-related genetic markers of adult male mouse sperm following busulfan treatment. Materials and Methods: Forty adult NMRI mice (30 ± 5 g) were divided...

1,7-1 Irpiatirti ¡ol d¡sull'amati-(hepsulfam, NSC 329680) is a new antileukemic agent with close structural similarity to busulfan. The mecha nism of action of hepsulfam is not known and it has recently been entered into Phase I clinical trials by the National Cancer Institute. Waud et al. have recently shown that hepsulfam has good antitumor activity against mouse 1.1210 leukemia in vivo (Wa...

Chemotherapeutic agent, busulfan, induce oxidative stress as a mechanism to kill cancer cells, however, it may also cause in non-target tissues and thereby lead normal tissue injury. Milk thistle (Silybum marianum L.) has been used for centuries herbal drug. The present study was carried out investigate the potential effects of wild Silybum seed ethanol extract (SMSEE) intervention on induced b...

Following a course of busulfan, mice sustain a residual injury to the marrow which is characterized by prolonged or permanent mild marrow hypoplasia and which frequently terminates in severe marrow aplasia and death. The stem cells and the marrow environment of mice bearing this residual injury were studied by assessing the ability of cells from these mice to grow when transplanted into irradia...

In a randomized multicenter study the influence of hydroxyurea versus busulfan on the duration of the chronic phase and on survival of chronic myelogenous leukemia (CML) was determined. In addition cross resistance and adverse reactions of the drugs were analyzed. From July 1983 to January 1991, 441 CML patients were randomized to receive hydroxyurea or busulfan. Of these, 90.7% were Philadelph...

Testicular cell transplantation has been widely used to investigate the biology of spermatogonial stem cells, production of transgenic animals, and restoration of fertility in rodent models. One critical step in successful transplantation is the preparation of the recipient testes. Busulfan has been widely used, but irradiation has been often suggested as an alternative. There have only been li...

The objective of this study was to externally validate a new dosing scheme for busulfan. Thirty-seven adult patients who received busulfan as conditioning therapy for hematopoietic stem cell transplantation (HCT) participated in this prospective study. Patients were randomized to receive intravenous busulfan, either as the conventional dosage (3.2 mg/kg daily) or according to the new dosing sch...

Busulfan liver metabolism depends on glutathione, a crucial mediator of cellular and systemic stress. Here we investigated 40 polymorphisms at 27 loci involved in hepatic glutathione homeostasis, with the aim of testing their impact on the clinical outcome of 185 busulfan-conditioned allogeneic transplants. GSTA2 S112T serine allele homozygosity is an independent prognostic factor for poorer su...

busulfan, a cytotoxic drug, which is currently used as a chemotherapeutic agent, has many side effects on different body organs. in this research, the effects of busulfan on sperm parameters and microstructure of mouse testis were investigated. methods: busulfan was injected intrapretoneally at 10, 20, 30, 40 and 50 mg/kg and testes were removed after 4, 6 and 8 weeks, weighed and processed for...

A national cooperative group trial was conducted in 153 patients with chronic myelogenous leukemia (CML) in chronic phase treated with oral pulse busulfan to determine if oral vitamin A can increase the time to blast crisis and enhance survival of patients. Patients diagnosed within 1 year and in the chronic phase of CML were randomized to receive oral pulse busulfan or the alkylator plus conti...