Glutaminase-asparaginase from Pseudomonas 7A appears to have four subunits with a molecular weight of 36,000 +/- 500 by sedimentation equilibrium in 5.9 M guanidine HCl and 34,000 by amino acid analysis. Analytic sedimentation equilibrium of the native enzyme showed a molecular weight of 140,000 +/- 3,300 with no signs of association or dissociation. Moving boundary and zone sedimentation in bu...

PURPOSE The aim of the study was the development of a simple and rapid analytical procedure for the determination of the most frequently used antihistamine derivatives. METHODS A capillary zone electrophoretic method was developed for the simultaneous separation of loratadine, desloratadine and cetirizine. Efforts were focused primarly on the optimisation of the experimental parameters: buffe...

This paper presents a model which provides a smooth transition between a kinetic and a diffusion domain. The idea is to use a buffer zone, in which both diffusion and kinetic equations will be solved. The solution of the original kinetic equation will be recovered as the sum of the solutions of these two equations. We use an artificial connecting function which makes the equation on each domain...

Shingled Magnetic Recording (SMR) technology increases the areal density of hard disk drives. Among the three types of SMR drives on the market today, Host Aware SMR (HA-SMR) drives look the most promising. In this paper, we carry out evaluation to understand the performance of HA-SMR drives with the objective of building large-scale storage systems using this type of drive. We focus on evaluat...

This paper presents a model which provides a smooth transition between a kinetic and a hydrodynamic domain. The idea is to use a buffer zone, in which both diffusion and kinetic equations will be solved. The solution of the original kinetic equation will be recovered as the sum of the solutions of these two equations. We use an artificial connecting function which makes the equation on each dom...

• The increasing tourism services near protected areas affect adversely the landscape. anthropization index was applied considering land cover of different years. tourist infrastructure is in most anthropized sites buffer zone.

Riparian buffer strips are a widely accepted tool for helping to sustain aquatic ecosystems and to protect downstream resources and values in forested areas, but controversy persists over how wide a buffer strip is necessary. The physical integrity of stream channels is expected to be sustained if the characteristics and rates of tree fall along buffered reaches are similar to those in undistur...

In this paper, we are evaluating the strategy of sorting peptides/proteins based on the charge to mass without resorting to ampholytes and/or isoelectric focusing, using a single- and two-step free-flow zone electrophoresis. We developed a simple fabrication method to create a salt bridge for free-flow zone electrophoresis in PDMS chips by surface printing a hydrophobic layer on a glass substra...