The object of this study was to identify the possible effects of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on cumulus expansion, nuclear maturation and fertilization of buffalo cumulus oocyte complexes (COC’s) matured and fertilized in vitro in two serum free media Dulbecco’s modified Eagles medium (DMEM) and Ham’s F-10. Oocytes were matured in vitro for 24 h either...

The present experiment has been conducted to study the post thaw morphology of buffalo oocytes vitrified at different stages of in vitro maturation (IVM) using propylene glycol as a cryoprotectant. Cumulus oocyte complexes (COCs) obtained from slaughterhouse ovaries were randomly divided into 6 different groups: control (nonvitrified oocytes were matured for 24 h in maturation medium (MM) consi...

The present study has evaluated the association of growth differentiation factor9 (GDF9) and bone morphogenetic protein15 (BMP15) mRNA expression in cumulus-oocyte complexes (COCs) of buffalo ovary during in vitro maturation (IVM). GDF9 and BMP15 are expressed specifically in mammalian oocytes and also participate in cumulus-oocyte crosstalk. Quantitative real-time polymerase chain reaction (qR...