We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...

oxidation reaction of indole to isatin was performed in the presence of fe3o4 nanoparticles (as catalyst) and commercial grade perhydrol (as oxidant). magnetite nanoparticles were synthesized via chemical precipitation method and characterized by xrd and tem techniques. the efficiency of reaction was obtained from gc analysis of the mixture. the catalyst was easily separated from the reaction m...

background: acanthamoeba keratitis develops by pathogenic acanthamoeba such as a. pal­es­tinen­sis . indeed this species is one of the known causative agents of amoebic keratitis in iran. mannose binding protein (mbp) is the main pathogenicity factors for developing this sight threatening disease. we aimed to characterize mbp gene in pathogenic acanthamoeba isolates such as a. palestinensis . m...

polymerase chain reaction and restriction fragment length polymorphism (pcr-rflp) and phylogenetic analysis were used for molecular identification of lactic acid bacteria (labs) isolated from apis mellifera. eighteen honeybee workers were collected from three different apiaries in west azerbaijan. labs from the gut of honeybees were isolated and cultured using routine biochemical procedures. ge...

The ovine melatonin receptor 1A (MTNR1A) and aromatase (CYP19) genes were structurally characterized and the association between their variants and reproductive and growth traits was studied in Kurdi sheep at Kurdi sheep breeding station located in Shirvan, Iran. The genomic DNA was extracted by guanidine thiocyanate-silica gel method. Polymerase chain reaction was carried out to amplify 824 bp...

conclusions using the pcr-rflp method, three distinctive h. pylori caga genotypes were detected in antral biopsies. genotype i, which was predominant among the isolates, was significantly associated with gastritis. however, the data showed that caga genotype iii may play a role in duodenitis and duodenal ulcers in patients infected with h. pylori. patients and methods a total of 161 gastric bio...

Eight oligonucleotide primers in 7 different combinations were used to amplify 3D gene sequences of foot-and-mouth disease virus (FMDV) by reverse transcription-polymerase chain reaction (RT-PCR). Six of the primers were designed at this laboratory. All the primer combinations could specifically amplify 3D gene sequences of FMDV serotypes O, A, and C. The largest fragment amplified was of 1,393...

The Brønsted acidic ionic liquid ([BMIm]HSO4) catalyzed three-component synthesis of 2H-indazolo[2,1-b]phthalazine-trione derivatives at room temperature. The integrity of the ionic liquid remains reasonably unchanged when it is separated from the reaction mixture by water extraction, as it can be recycled several times without any loss of activity in each of the title syntheses. The products a...