Here we report, novel amphiphilic fluorescent probes for selective detection of BSA (bovine serum albumin), via non-covalent interactions. The intrinsic weak fluorescence of the probes in an aqueous medium increased dramatically after addition of BSA, while responses to various other proteins/enzymes are negligible.

In this work, we present a methodology for choosing the best experimental conditions for transferring protein Langmuir films onto solid substrates. As an example of applying the proposed methodology, we used monolayers of the protein bovine serum albumin, which is a very stable protein and is of great interest in the development of immunosensors. Langmuir-Blodgett (LB) films of this protein, on...

The Standards Committee of the AACC presents a discussion of the nature of total serum protein and the problems associated with its determination. Proposals are made that protein be defined as polypeptide material, and that a reference preparation be promuJgted for interlaboratory use. The reference material suggested is bovine serum albumin, produced to rigid specifications and distributed as ...

Steady-state conductivity measurements and dielectric measurements in the frequency range 10(-5) to 100 Hz are reported for samples of bovine serum albumin, casein, and lysozyme complexed with methylglyoxal. Compared with the untreated proteins, the brown complexed proteins exhibit an increased conductivity and free electron spin density, together with a low-frequency dielectric dispersion. The...

Fourier transform infrared (FT-IR) spectra have been measured for defatted bovine serum albumin (BSA) in D(2)O with a concentration of 2.0 wt % over a temperature range of 26-90 degrees C and the corresponding difference spectra have been calculated by subtracting the contribution of D(2)O at the same temperature. Evolving factor analysis (EFA) by selecting two factors and three factors has bee...

Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker lea...

There are some theoretical arguments related to interpreting the adiabatic compressibility (beta(s)) of a protein determined from the sound velocity and the difference between beta(s) and isothermal compressibility (beta(T)). To address these problems experimentally, we constructed a high-pressure oscillating densitometer and used it to measure the apparent specific volume of bovine serum album...

To evaluate the re lat ionship be tween tumor burden and c i rculat ing immune complexes (IC) in ch i ldren with neurob lastoma (NBL), we studied sera col lected at intervals from pat ients with d isseminated (Stage III or IV) NBL. Sera from 10 of 12 pat ients conta ined IC by the Raji cell assay at some time dur ing the first 9 to 11 months of the study. H igher IC levels were observed in sera...