bovine leukosis virus (blv) is a retrovirus that primarily affects lymphoid tissue of cattle. to determine the seroprevalence of enzootic bovine leukosis virus infection and its effects on some clinical, hematological and flow cytometric indications, this study was conducted on 197 holstein cattle slaughtered in an abattoir in tehran province. elisa gp51 test of the obtained serums of the anima...

Biotechnological methods offer promising approaches for improved diagnostic and prophylactic purposes. The following biotechnological techniques are used in the Institute of Virology at the Hanover Veterinary School:--Production of monoclonal antibodies directed against viral and bacteria-specific antigens such as bovine virus diarrhoea virus, classical swine fever (hog cholera) virus, feline l...

in order to estimate the mean of maternal antibody in calves against bhv-1 (bovine herpes virus type 1), this study was carried out in a population of calves from non-vaccinated dairy cattle at 2 livestock in qazvin province. one hundred thirteen sera out of 512 were collected from 1-4 months unvaccinated calves. we used blocking –percentage of maternal antibodies against bhv-1, which obtained ...

In situ PCR was developed for the detection of proviral DNA of bovine leukaemia virus in paraffin embedded tissues, tissue smears of experimentally inoculated sheep, as well in peripheral blood leukocytes of naturally infected cattle. The liver, spleen, lung, heart, kidney, and lymph node were collected from two infected sheep, which died or were euthanized due to lymphosarcoma and persistent l...

Investigations were performed in lambs experimentally infected with blood lymphocytes of leukaemic cow. The presence of specific bovine leukaemia virus (BLV) antibodies in sera of infected animals was detected in agar gel immunodiffusion test and ELISA. Proviral DNA was detected with PCR and nested PCR. Dual-colour flow cytometry analysis with the use of specific monoclonal antibodies (for lymp...