Here the transcriptome of an oil-rich race B strain of Botryococcus braunii (BOT-70) was analyzed to mine genetic information useful in biofuel development. A full-length-enriched cDNA library was constructed via the oligo-capping method and the 5' ends of 11,904 randomly chosen cDNA clones were sequenced. Homology search using BLASTX identified candidate BOT-70 genes for majority of the reacti...

The key parameters of the inner structure of a cylindrical airlift bioreactor, including the ratio of the cross-section area of the downcomer to the cross-section area of the riser, clearance from the upper edge of the draft tube to the water level, and clearance from the low edge of the draft tube to the bottom of the reactor, significantly affected the biomass production of Botryococcus braun...

Optimization of the light conditions for biofuel production by the microalga Botryococcus braunii BOT-22 (race B) was performed using monochromatic red light. The lipid and sugar contents were approximately 40% and 20-30% of the cell dry weight, respectively, and about half of the lipids were liquid hydrocarbons. The half-saturation intensities for the production rate of lipids, hydrocarbons, a...

Skim is a major by-product of the virgin coconut oil (VCO) industry consisting nutrients and currently discharged to environment as waste. Hence, this research aimed utilize skim medium for Botryococcus braunii Chlorella vulgaris growth. The effect organic carbon resourced from glucose glycerol on biomass was also investigated at certain concentrations with intervals 100:0 0:100 (%). Results in...