نتایج جستجو برای: blots
تعداد نتایج: 5091 فیلتر نتایج به سال:
We introduce here an alternative to conventional northern blotting that requires only minute amounts of RNA. This has been achieved by modification of methods currently used for the mapping of mRNA 5'-terminal ends. The terminal desoxynucleotidyl transferase-mediated G-tailing, cap finder, ligation-anchored and RNA ligase-mediated approaches followed by polymerase chain reaction protocols all p...
The guinea pig sperm surface protein PH-20 has an essential function in sperm adhesion to the zona pellucida of guinea pig eggs. Fully effective contraception has been achieved by immunizing either male or female guinea pigs with purified guinea pig PH-20. Here we report the isolation of human and cynomolgus monkey PH-20 cDNAs as a key step toward testing the function of primate PH-20 and the c...
Retraction of: J. Cell Sci. 118, 1629-1639. We were recently made aware of errors in our paper, which include misrepresentation of western blot data in Figs 4, 5 and 6 as detailed below. The misuse and re-use of western blot bands breaches the editorial policy of Journal of Cell Science, and so we must retract this article. The corresponding author, A.S., regrets the inappropriate figure manipu...
Rhodococcus I24 and B264-1 are able to stereospecifically bioconvert indene to indandiol,a potential precursor to CRIXIVAN. Indene bioconversion is catalyzed by the multicomponent enzymes, naphthalene d ioxygenase and toluene dioxygenase. The genes for these enzy mes have not been identified in Rhodococcus, but sequences are known in Pseudomonas. The objective of these experiments is to screen ...
Genes encoding the four principal polypeptide domains (N, A1-B1, A2-B2, and A3-B3) of carcinoembryonic antigen (CEA) were synthesized and expressed in Escherichia coli as fusion products with bacterial CMP-KDO synthetase (CKS). The four synthetic fusion proteins were purified in high yield and used as targets in Western blots for 11 anti-CEA MAbs and to compete with immobilized CEA for binding ...
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