purpose: to determine bacterial contamination of corneas and corneal storage media provided from the eye bank of i.r. iran to rasool akram medical center. methods: this descriptive study was performed on 70 corneoscleral rims and corneal storage media. one milliliter of corneal storage media and the corneoscleral rim were sent to microbiology laboratory at the time of penetrating keratoplasty a...

BACKGROUND Well-characterized biochemical, structural, and physiological changes occur when red blood cells (RBCs) are stored for a period of time and are collectively called the storage lesion. METHODS Key study results are summarized and contrasted and new data from recently completed randomized controlled trials will be discussed. RESULTS It is unclear whether in vitro changes to RBCs th...

Red blood cells (RBCs) undergo biochemical and structural changes during storage, commonly referred to as the "storage lesion." Evidence suggests that the longer the RBC product is stored, the less effective is the transfused blood. Many studies linking morbidity to transfusion have not considered duration of RBC storage as a variable that may modulate the effect. In addition, the effects of su...

BACKGROUND The aim of this study was to evaluate the optimal preanalytical conditions prior to nucleic acid amplification technology (NAT) for human immunodeficiency virus-1 (HIV-1) or Hepatitis C virus (HCV) RNA in pools of 96 plasma specimens with regard to storage temperature, time and plasma separation in a blood donation environment. STUDY DESIGN AND METHODS Changes in viral nucleic acid...

A method is described for preservation of blood for delayed determinations of ammonia by rapid freezing of the samples in a dry ice and acetone solution followed by storage of the specimens in the frozen state. The method was evaluated by first establishing the reproducibility of the chemical method used for blood ammonia analysis in 714 consecutive samples of dog blood which were analyzed in d...

Depending on logistics, whole blood has to be stored for several hours after collection. If storage time exceeds 8 h, storage has to be at 1-6 degrees C to comply with FDA regulations. In the Netherlands, however, whole blood is also stored for 12-15 h at 20-24 degrees C using butane-1,4-diol cooling devices. We compared these two storage methods for factor VIII recovery in plasma and cryopreci...

Blood samples used in hemorheological studies may be stored for a period of time, the effects of storage have yet to be fully explored. This study evaluated the effects of storage temperature (i.e., 4 degrees C or 25 degrees C) and duration on RBC deformability and aggregation for blood from healthy controls and from septic patients. Our results indicate that for normal blood, RBC deformability...

to assess the effects of pre-analytical handling (storage time and temperature) on selected hematological parameters, whole blood samples were collected in edta coated tubes from each of 30 clinically normal male adult beagle dogs. each sample was separated in 2 aliquots, of which one was stored in ambient temperature (25 ˚c) and the other one was refrigerated (2 to 4 ˚c). complete blood counts...