With a video-rate third harmonic generation (THG) microscopy system, we imaged the micro-circulation beneath the human skin without labeling. Not only the speed of circulation but also the morpho-hydrodynamics of blood cells can be analyzed. Lacking of nuclei, red blood cells (RBCs) shows typical parachute-like and hollow-core morphology under THG microscopy. Quite different from RBCs, every no...

The heart drives two separate and distinct circulatory systems in the body: the pulmonary circulation and the systemic circulation. The pulmonary circulation is analogous to the entire systemic circulation. Similar to the systemic circulation, the pulmonary circulation receives all of the cardiac output. Therefore, the pulmonary circulation is not a regional circulation like the renal, hepatic,...

or saline and then administered cocaine (10 mg/kg, i.v. bolus). Coronary blood flow, heart rate, mean arterial pressure, and the first derivation of left ventricular pressure (dP/dt) were measured at baseline, 2 minutes, and 15 minutes after cocaine administration. Nifedipine pretreatment prevented the early cocaine-induced decrease in coronary blood flow and improved left ventricular dP/dt com...