The ability of Shigella flexneri to interact with lactoferrin (Lf) was examined with a 125I-labeled protein-binding assay. The percent binding of human lactoferrin (HLf) and bovine lactoferrin (BLf) to 45 S. flexneri strains was 19 +/- 3 and 21 +/- 3 (mean +/- standard error of the mean), respectively. 125I-labeled HLf and BLf binding to strain M90T reached an equilibrium within 2 h. Unlabeled ...

INTRODUCTION Singing for Lung Health (SLH) groups are an increasingly popular intervention for people with respiratory disease. There are limited data as to how these groups should be developed and run. We aimed to evaluate the experience of singing leaders both to assess the training provided by the British Lung Foundation (BLF) and to provide information to guide future development of program...

Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 strains are associated with haemorraghic colitis and haemolytic uremic syndrome (HUS) in humans. Cattle are a reservoir of E. coli O157:H7. We studied the ability of bovine and human lactoferrin, two natural antimicrobial proteins present in milk, to inhibit E. coli O157:H7 growth and attachment to a human epithelial colorectal adenocar...

As proposed by Ameka and Levinson (this issue) locative verb systems can be classified into four types according to the number of verbs distinguished. This article addresses the lower extreme of this typology: languages which o¤er no choice of verb in the basic locative function (BLF). These languages have either a single locative verb, or do not use verbs at all in the basic locative construct...

Transforming growth factor (TGF)-beta plays a central role in lung fibrosis, stimulating extracellular matrix deposition. Intracellular signaling of TGF-beta is mediated by Smad proteins. We questioned whether the expression and activation of Smads would be altered in lung fibroblasts from rats exposed to bleomycin, an agent used to provoke an experimental model of lung fibrosis. Fibroblasts we...

BACKGROUND Inadequate sleep may aggravate hypertension, but the pathophysiology of sleep disturbance in hypertension remains unknown. Among spontaneously hypertensive rats (SHR), sleep disturbance co-occurred with sympathetic hyperactivity; therefore, we hypothesized that the sleep disturbance can be alleviated by antagonizing the adrenergic overdrive. METHODS Polysomnographic recording was p...

BACKGROUND Human milk provides necessary macronutrients (protein, carbohydrate, fat) required for infant nutrition. Lactoferrin (Lf), a multifunctional iron-binding protein predominant in human milk, shares similar protein sequence, structure, and bioactivity with bovine Lf (bLf). This large-scale pediatric nutrition study was designed to evaluate growth and tolerance in healthy infants who rec...

Supported by: CNPq #910149/96-98 and NSF Int-9604599 to LCLS and BLF and NSF Grant IBN-0138113 to BLF. Multiple people contributed to the long term project that includes this paper. We thank Justin Crowley, Elizabeth Yamada, Michael Hersman and David Ziegler for their help with the initial phases of this project, Francinaldo Lobato Gomez, Walter Augusto de Carvalho, Edna S. Franco, and Cezar Ak...

This article investigates the adaptive fuzzy control design for p-norm stochastic high-order lower triangular nonlinear systems with output constraints and unknown nonlinearities. First of all, a tan-type barrier Lyapunov function (BLF) is constructed to deal constraint issue. Subsequently, an algorithm developed by combining BLF adding power integrator technique. Simultaneously, analysis shows...

Interaction of lactoferrin (Lf) with the cell envelope (CE) and outer membrane (OM) of Salmonella typhimurium-type strain ATCC13311 was tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western-blot analyses. The peroxidase-labeled bovine Lf (BLf) and human Lf both recognized a heat-modifiable protein with an estimated molecular mass of 38 kD in the OM. Simultaneous immuno...