We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau of Standards) as the standard. At 25 degrees C, color development for 30 or 60 min provides identical serum protein values. Gluco...

C ORN silage is a major source of roughage for ruminants in the cornbelt, because it is one of the most productive crops of total digestible nutrients and animal gains per acre. However, to make up a complete growingfinishing ration with corn silage it is necessary to supplement it with sources of energy, nitrogen and minerals. The addition of urea to corn, sorghum or grass at ensiling has give...

Determination of total urinary protein is now more frequently requested of the clinical chemistry laboratory, one reason being the increased number of patients with renal transplants for whom total urinary protein is determined as an index of immunological rejection (1). Available manual methods are cumbersome and inefficient under clinical laboratory conditions. Concentrations for total urinar...

An Fe(III) complex of a biuret-amide based macrocyclic ligand that exhibits both excellent reactivity for the activation of H(2)O(2) and high stability, especially at low pH and high ionic strength, is reported.

From these results it is permissible to draw the following conclusions which may be used to explain certain phenomena associated especially with the onset of acute pancreatitis. 1. The sudden marked collapse of acute pancreatitis has as its most important cause a toxin or toxins derived from broken down pancreatic tissue. This toxin or toxins seem to be most powerful in the stage of degradation...

A kinetic model for urea decomposition and byproduct formation was developed validated against thermogravimetric analyses (TGA) of urea-derived byproducts, including biuret cyanuric acid, at heating rates up to 500 °C/min. The provided a good description all investigated rates. results indicate that an increase in rate causes shift production acid into ammelide limits the overall formation. Add...

The quantitative determination of proteins in biological fluids, using Coomassie Brilliant Blue G-250, was evaluated. Compared with the biuret method, the Coomassie Blue G-250 method needs a much shorter time for analysis and has a greater sensitivity. The sensitivity of the dye for albumin is significantly greater than for globulins. The standard curves for the biuret method are more linear th...

In this new method for determining urinary protein, the reaction is complete within 10 min at 37 degrees C. This method is applicable to automated as well as manual measurements. Protein concentration and absorbance at 600 nm are linearly related throughout a wide range of concentrations, 10 to 16 000 mg/L. However, the chromogenicity of the gamma-globulins in this method is 70% of that of albu...