نتایج جستجو برای: biosynthetic gene clusters

تعداد نتایج: 1274616  

2015
Tilmann Weber Kai Blin Srikanth Duddela Daniel Krug Hyun Uk Kim Robert E. Bruccoleri Sang Yup Lee Michael A. Fischbach Rolf Müller Wolfgang Wohlleben Rainer Breitling Eriko Takano Marnix H. Medema

Microbial secondary metabolism constitutes a rich source of antibiotics, chemotherapeutics, insecticides and other high-value chemicals. Genome mining of gene clusters that encode the biosynthetic pathways for these metabolites has become a key methodology for novel compound discovery. In 2011, we introduced antiSMASH, a web server and stand-alone tool for the automatic genomic identification a...

Journal: :Applied and environmental microbiology 2004
Michelle C Moffitt Brett A Neilan

Nodularia spumigena is a bloom-forming cyanobacterium which produces the hepatotoxin nodularin. The complete gene cluster encoding the enzymatic machinery required for the biosynthesis of nodularin in N. spumigena strain NSOR10 was sequenced and characterized. The 48-kb gene cluster consists of nine open reading frames (ORFs), ndaA to ndaI, which are transcribed from a bidirectional regulatory ...

2016
Thomas C McLean Paul A Hoskisson Ryan F Seipke

Streptomyces species produce an incredible array of high-value specialty chemicals and medicinal therapeutics. A single species typically harbors ~30 biosynthetic pathways, but only a few them are expressed in the laboratory; thus, poor understanding of how natural-product biosynthesis is regulated is a major bottleneck in drug discovery. Antimycins are a large family of anticancer compounds wi...

2015
Michalis Hadjithomas I-Min Amy Chen Ken Chu Anna Ratner Krishna Palaniappan Ernest Szeto Jinghua Huang T. B. K. Reddy Peter Cimermančič Michael A. Fischbach Natalia N. Ivanova Victor M. Markowitz Nikos C. Kyrpides Amrita Pati

UNLABELLED In the discovery of secondary metabolites, analysis of sequence data is a promising exploration path that remains largely underutilized due to the lack of computational platforms that enable such a systematic approach on a large scale. In this work, we present IMG-ABC (https://img.jgi.doe.gov/abc), an atlas of biosynthetic gene clusters within the Integrated Microbial Genomes (IMG) s...

Journal: :Cell 2014
Peter Cimermancic Marnix H. Medema Jan Claesen Kenji Kurita Laura C. Wieland Brown Konstantinos Mavrommatis Amrita Pati Paul A. Godfrey Michael Koehrsen Jon Clardy Bruce W. Birren Eriko Takano Andrej Sali Roger G. Linington Michael A. Fischbach

Although biosynthetic gene clusters (BGCs) have been discovered for hundreds of bacterial metabolites, our knowledge of their diversity remains limited. Here, we used a novel algorithm to systematically identify BGCs in the extensive extant microbial sequencing data. Network analysis of the predicted BGCs revealed large gene cluster families, the vast majority uncharacterized. We experimentally...

2016
Shradha Khater Swadha Anand Debasisa Mohanty

In silico methods for linking genomic space to chemical space have played a crucial role in genomics driven discovery of new natural products as well as biosynthesis of altered natural products by engineering of biosynthetic pathways. Here we give an overview of available computational tools and then briefly describe a novel computational framework, namely retro-biosynthetic enumeration of bios...

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