Lactoperoxidase is an enzyme that contributes to the antimicrobial defense in secretory fluids and that has attracted interest as a potential biopreservative for foods and other perishable products. Its antimicrobial activity is based on the formation of hypothiocyanate (OSCN-) from thiocyanate (SCN-), using H2O2 as an oxidant. To gain insight into the antibacterial mode of action of the lactop...

Because of their antagonistic activity towards pathogenic and spoilage bacteria, some members of the lactic acid bacteria (LAB) have been evaluated for use as food biopreservatives. The objectives of this study were to assess the antimicrobial utility of a commercial LAB intervention against O157 and non-O157 Shiga-toxigenic E. coli (STEC) on intact beef strip loins during refrigerated vacuum a...

In the present study, a bacterium isolated from Marcha- a herbal cake used as traditional starter culture to ferment local wine in North East India, was evaluated for bacteriocin like inhibitory substance production and was tested against six food borne/spoilage causing pathogens viz. Listeria monocytogenes MTCC 839, Bacillus subtilis MTCC 121, Clostridium perfringens MTCC 450, Staphylococcus a...

In this paper, the antibacterial effects of the Bacillus amyloliquefaciens-produced bacteriocin subtilosin, both alone and in combination with curcumin, ε-poly-L-lysine (poly-lysine), or zinc lactate, were examined against Listeria monocytogenes. Results indicated that subtilosin inhibits both of the studied bacterial strains, Scott A (wild-type, nisin sensitive) and NR30 (nisin resistant). How...

Listeria monocytogenes is a foodborne pathogen associated with fermented products. The aim of this study was to characterize anti-listerial bacteriocin produced by lactic acid bacteria (LAB) isolated from Cambodian fermented foods. Four LAB isolates of confirmed bacteriocin production were isolated from fermented fish and shrimp (pha ak trey, pha ak kampus). These isolates demonstrated antimicr...

26 27 Phage-encoded endolysins are recently considered as new biocontrol tools to inhibit 28 pathogens in food. In this work, we have studied the ionic requirements for optimal lytic 29 activity of LysH5, the endolysin encoded by the staphylococcal bacteriophage phi30 SauS-IPLA88. LysH5 activity was inhibited by the presence of Mn and Zn and 31 enhanced by Ca, Mg and NaCl. When LysH5 was combin...