This article presents a novel SPR imaging biomolecular interaction detection method based on time domain phase modulation. An experimental apparatus of SPR imaging biomolecular interaction detection based on TDPM is established to detect biomolecular interaction. During the experimental pretreatment process, we prepared the 2×2 lysozyme array chip and detected lysozyme thermal condensation stat...

In literature, most researches related to the understanding of the behaviour of the cell networks focus only on some parts of the biomolecular network. However, to completely understand their behaviour over time we have to integrate the different parts of the biomolecular network and analyse them together. The objective of the present study is to propose to the biologist a platform to simulate ...

Decomposition of biomolecular reaction networks into pathways is a powerful approach to the analysis of metabolic and signalling networks. Current approaches based on analysis of the stoichiometric matrix reveal information about steady-state mass flows (reaction rates) through the network. In this work, we show how pathway analysis of biomolecular networks can be extended using an energy-based...

Single-molecule detection (SMD) has demonstrated some attractive benefits for many types of biomolecular analyses including enhanced processing speed by eliminating processing steps, elimination of ensemble averaging and single-molecule sensitivity. However, it's wide spread use has been hampered by the complex instrumentation required for its implementation when using fluorescence as the reado...

Multidimensional NMR spectroscopy is a well-established technique for the characterization of structure and fast-time-scale dynamics of highly populated ground states of biological macromolecules. The investigation of short-lived excited states that are important for molecular folding, misfolding and function, however, remains a challenge for modern biomolecular NMR techniques. Off-equilibrium ...

Biomolecular compounds, such as proteins and nucleic acids, which are evolutionary optimized, with respect to specificity of binding to their target structure as well as to functionality, for distinct biochemical transformation and translocation, are currently explored as building blocks in the ™bottom-up∫ self-assembly of nanometer-scale functional devices. [1] So far, applications include the...