نتایج جستجو برای: biolayer interferometry

تعداد نتایج: 11333  

Journal: :Biochimica et biophysica acta 2012
Stéphane L Benoit Jonathan L McMurry Stephanie A Hill Robert J Maier

BACKGROUND The gastric pathogen Helicobacter pylori relies on nickel-containing urease and hydrogenase enzymes in order to colonize the host. Incorporation of Ni(2+) into urease is essential for the function of the enzyme and requires the action of several accessory proteins, including the hydrogenase accessory proteins HypA and HypB and the urease accessory proteins UreE, UreF, UreG and UreH. ...

2014
Spandana Makeneni Ye Ji David C. Watson N. Martin Young Robert J. Woods

The ABO blood group system is the most important blood type system in human transfusion medicine. Here, we explore the specificity of antibody recognition toward ABO blood group antigens using computational modeling and biolayer interferometry. Automated docking and molecular dynamics simulations were used to explore the origin of the specificity of an anti-blood group A antibody variable fragm...

2017
Mayilaadumveettil Nishana Namrata M Nilavar Rupa Kumari Monica Pandey Sathees C Raghavan

Integrase inhibitors are a class of antiretroviral drugs used for the treatment of AIDS that target HIV integrase, an enzyme responsible for integration of viral cDNA into host genome. RAG1, a critical enzyme involved in V(D)J recombination exhibits structural similarity to HIV integrase. We find that two integrase inhibitors, Raltegravir and Elvitegravir, interfered with the physiological func...

Journal: :Biotechnology and bioengineering 2017
Venkata Giridhar Poosarla Tong Li Boon Chong Goh Klaus Schulten Thomas K Wood Costas D Maranas

Antibody drugs play a critical role in infectious diseases, cancer, autoimmune diseases, and inflammation. However, experimental methods for the generation of therapeutic antibodies such as using immunized mice or directed evolution remain time consuming and cannot target a specific antigen epitope. Here, we describe the application of a computational framework called OptMAVEn combined with mol...

2014
Megen A. Culpepper Amy C. Rosenzweig

In the initial steps of their metabolic pathway, methanotrophic bacteria oxidize methane to methanol with methane monooxygenases (MMOs) and methanol to formaldehyde with methanol dehydrogenases (MDHs). Several lines of evidence suggest that the membrane-bound or particulate MMO (pMMO) and MDH interact to form a metabolic supercomplex. To further investigate the possible existence of such a supe...

Journal: :Journal of biomolecular techniques : JBT 2012
Satya P Yadav Simon Bergqvist Michael L Doyle Thomas A Neubert Aaron P Yamniuk

The field of label-free biophysical technologies used to quantitatively characterize macromolecular interactions with each other and with small molecules has grown enormously in the last 10 years. The most widely used analytical technologies for characterizing biomolecular interactions are surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), biolayer interferometry (BLI), an...

2016
Adriana Badarau Harald Rouha Stefan Malafa Michael B. Battles Laura Walker Nels Nielson Ivana Dolezilkova Astrid Teubenbacher Srijib Banerjee Barbara Maierhofer Susanne Weber Lukas Stulik Derek T. Logan Martin Welin Irina Mirkina Clara Pleban Gerhild Zauner Karin Gross Michaela Jägerhofer Zoltán Magyarics Eszter Nagy

LukGH (LukAB) is a potent leukocidin of Staphylococcus aureus that lyses human phagocytic cells and is thought to contribute to immune evasion. Unlike the other bi-component leukocidins of S. aureus, LukGH forms a heterodimer before binding to its receptor, CD11b expressed on professional phagocytic cells, and displays significant sequence variation. We employed a high diversity human IgG1 libr...

2015
QIANG YOU YAN WU NANNAN YAO GUANNAN SHEN YING ZHANG LIANGGUO XU GUIYING LI CYNTHIA JU

Apoptosis inhibitor of macrophages (AIM/cluster of differentiation 5 antigen-like/soluble protein α) has been shown to inhibit cellular apoptosis; however, the underlying molecular mechanism has not been elucidated. Using yeast two‑hybrid screening, the present study uncovered that AIM binds to insulin‑like growth factor binding protein‑4 (IGFBP‑4). AIM interaction with IGFBP‑4, as well as IGFB...

Journal: :Journal of immunology 2011
Jonathan B Rothbard Xiaoyan Zhao Orr Sharpe Michael J Strohman Michael Kurnellas Elizabeth D Mellins William H Robinson Lawrence Steinman

For 15 y, α B-crystallin (heat shock protein [Hsp] B5) has been labeled an autoantigen in multiple sclerosis (MS) based on humoral and cellular responses found in humans and animal models. However, there have been several scientific inconsistencies with this assignment, ranging from studies demonstrating small differences in anticrystallin responses between patients and healthy individuals to t...

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