Hashimoto’s patients have in some cases both stimulating and blocking antibodies [2, 3]. It stands to reason that if the patient is hypothyroid, then blocking antibody avidity and/ or titer would dominate irrespective of the presence of stimulating antibodies. There is a growing list of publications that show the prevalence of TSHR stimulating autoantibodies in HT patients using bioassays. Wall...

Aedes aegypti L. is the mosquito vector of yellow fever, dengue, zika, and chikungunya viruses. The prevention control such diseases usually rely on use chemicals, that can cause harm to human health environment. Vegetable oils with larvicidal activity are used as an alternative tool this insect. This study aimed evaluate vegetable from Caryocar coriaceum, Mauritia fIexuosa, Carapa guianensis, ...

BACKGROUND Variation in response to insecticidal proteins is common upon repetition of insect bioassays. Understanding this variation is a prerequisite to detecting biologically important differences. We tracked neonate Spodoptera frugiperda (J.E. Smith) susceptibility to Vip3Aa19 over 17 generations using standardized bioassay methods. Five larval pretreatment conditions and one bioassay condi...

Abstract Background and Aims: The Cydia pomonella granulovirus (CpGV) is a baculovirus and very effective biological control agent against apple pest, codling moth, Cydia pomonella L. especially for healthy organic apple production and protection.  Not only, screening of CpGV isolates via biochemical variation, but also their biological variation is practically necessary.  Howeve...

In pharmaceutical drug discovery and agricultural crop product discovery, in vivo bioassay experiments are used to identify promising compounds for further research. The reproducibility and accuracy of the bioassay is crucial to be able to correctly distinguish between active and inactive compounds. In the case of agricultural product discovery, a replicated dose-response of commercial crop pro...

Currently, the only accepted assay with which to detect active Clostridium botulinum neurotoxin is an in vivo mouse bioassay. The mouse bioassay is sensitive and robust and does not require specialized equipment. However, the mouse bioassay is slow and not practical in many settings, and it results in the death of animals. Here, we describe an in vitro cleavage assay for SNAP-25 (synaptosome-as...

Establishment of the nitrogen-fixing nodulation symbiosis between legumes and rhizobia requires plant-wide reprogramming to allow infection and development of nodules. Nodulation is regulated principally via a mechanism called autoregulation of nodulation (AON). AON is dependent on shoot and root factors and is maintained by the nodulation autoregulation receptor kinase (NARK) in soybean. We de...