An experiment was conducted to investigate the effect of dietary Ca level on the efficacy of phytase. A total of 288 male Ross × Ross 708 broilers with initial and final BW of 37 and 705 g, respectively, were used in brooder batteries from 0 to 21 d posthatch. Each treatment had 8 replications with 6 broilers/replicate pen. All diets were corn-soybean meal based and formulated to contain 1.26% ...

Three experiments were conducted to evaluate the effectiveness of OptiPhos phytase and citric acid for releasing the P that is not bioavailable in distillers dried grains with solubles (DDGS). The effect of OptiPhos phytate on AME(n) and amino acid digestibility was also determined. New Hampshire x Columbian chicks were fed experimental diets from 8 to 21 d of age. For Experiment 1, a basal P-d...

Partisphere SAX HPLC analysis of endogenous inositol phosphates in [3H]inositol-labelled barley aleurone tissue revealed a range of isomers, including D- and/or L-Ins3P, D- and/or L-Ins(1,4)P2, D- and/or L-Ins(1,2)P2, a third unidentified InsP2, Ins(1,2,3)P3, D- and/or L-Ins(1,2,6)P3, D-and/or L-Ins(1,2,3,4)P4, D- and/or L-Ins(1,2,5,6)P4, Ins(1,3,4,5,6)P5, D- and/or L-Ins(1,2,3,4,5)P5, Ins(1,2,...

A six wk feeding experiment was conducted with d-old four hundred broiler chicks (Ven Cobb) to determine the effects of microbial phytase (Allzyme) supplementation in soybean meal based broiler diet containing low phosphorous. These birds were randomly divided into four dietary treatment groups of 100 broilers each. Each treatment group was further sub-divided into five replicates of 25 broiler...

AIMS To isolate, clone and express a novel phytase gene (phy) from Bacillus sp. in Escherichia coli; to recover the active enzyme from inclusion bodies; and to characterize the recombinant phytase. METHODS AND RESULTS The molecular weight of phytase was estimated as 40 kDa on SDS-polyacrylamide gel electrophoresis. A requirement of Ca(2+) ions was found essential both for refolding and activi...

A total of 364 female Ross 308 chicks (1 d old) were used in the present study conducted in floor pens to investigate the effects of graded levels of supplementary bacterial phytase on dietary energy utilisation and growth performance. For this purpose, four maize-soyabean-based diets were offered to the birds from 0 to 21 d of age. These included a suboptimal P negative control (NC, 3.0 g/kg n...

Hens were fed corn-soybean meal diets containing 0.35, 0.25, 0.15, or 0.10% nonphytate phosphorus (NPP) (40 to 60 wk). Phytases A and B were added at 0.25, 0.15, and 0.10% at 250 to 300 units of phytase (FTU)/kg feed in a 3 x 3 factorial; 0.35% was a control diet. Treatments were replicated with eight cages per treatment (five hens per cage) in a randomized complete block design. Phytase supple...

The residual phytase activity, phytic acid P content, and microstructure of the digesta along the gastrointestinal tract (GIT) of broiler chickens fed a corn expressing microbial phytase was studied in a 14-d experiment. The phytase activity of the corn expressing phytase (CBP) was determined to be 660 phytase units/g and was incorporated into broiler diets at varying rates. One hundred forty-f...

Two experiments were conducted to determine the interactive effects of phytase with and without a trace mineral premix (TMP) in diets for nursery, growing, and finishing pigs on growth performance, bone responses, and tissue mineral concentrations. Pigs (initial and final BW of 5.5 and 111.6 kg [Exp. 1] or 5.4 and 22.6 kg [Exp. 2]) were allotted to treatments on the basis of BW with eight (Exp....

Phytase is well studied and explored, however, little is known about its effects on the microbial ecology of the gastrointestinal tract. In total, 400 one-day-old female Ross 308 chicks were randomly distributed to four experimental groups. The dietary treatments were arranged as a 2 × 2 complete factorial design, with the factors being adequate (PC) or insufficient calcium (Ca) and digestible ...