Our laboratory has previously developed a qRT-PCR assay to assess drug efficacy on the growth of Cryptosporidium parvum in vitro by detecting the levels of parasite 18S rRNA. This approach displayed up to four orders of magnitude of linear dynamic range and was much less labor-intensive than the traditional microscopic methods. However, conventional qRT-PCR protocol is not very amendable to hig...

Twenty-two infectious bursal disease virus (IBDV) strains were examined using the reverse transcriptase/polymerase chain reaction-restriction endonuclease (RT/PCR-RE) assay. A 394-bp fragment of the VP2 gene was amplified and tested for six different restriction enzyme sites. Although the EcoRII enzyme was used in previous RT/PCR-RE assays, results obtained using the isoschizomer BstNI were mor...

Assays to simultaneously detect multiple potential agents of bioterrorism are limited. Two multiplex PCR and RT-PCR enzyme hybridization assays (mPCR-EHA, mRT-PCR-EHA) were developed to simultaneously detect many of the CDC category "A" bioterrorism agents. The "Bio T" DNA assay was developed to detect: Variola major (VM), Bacillus anthracis (BA), Yersinia pestis (YP), Francisella tularensis (F...

UNLABELLED Transposon insertion sequencing is a high-throughput technique for assaying large libraries of otherwise isogenic transposon mutants providing insight into gene essentiality, gene function and genetic interactions. We previously developed the Transposon Directed Insertion Sequencing (TraDIS) protocol for this purpose, which utilizes shearing of genomic DNA followed by specific PCR am...

BACKGROUND The sensitivity and selectivity of traditional methods limits ultramicro detection of proteins. Bio-barcode amplification detection methods based on nanotechnology enables ultramicro detection of protein. However, bio-barcode amplification detection depends on the oligonucleotides being fixed on a glass chip. It also requires specialized equipment, which limits its application. We in...

statement of problem: the guided bone regeneration (gbr) technique does not always produce consistent results. bone filling within the space provided by the membrane can be incomplete. purpose: the aim of this study was to evaluate the effect of a collagen membrane (bio-gide) in combination with or without deproteinezed bovine bone mineral (bio-oss) on the healing of calvarial defects in rabbit...

Background: Polymerase chain reaction (PCR) methods from direct specimen are widely used for the rapid and accurate detection of mycobacteria infection. In this study, we evaluated two domestically developed detection kits for Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) using real-time PCR. Methods: A total of 348 samples from patients with suspected tuberculosis were...

Plant pathogens present a serious threat to seedling establishment and the potential for plant disease epidemics under greenhouse conditions is great. Hence, pathogen exclusion by detection and elimination of infested seedlots remains a requisite tactic for seedling production and disease management. Unfortunately, the numbers of contaminated seed within a lot may be low and infested seed may b...