​Background: Consumption of Lactobacillus paracasei Lpc-37 or Bifidobacterium lactis HN019 by 2-5-year-old children was found to reduce risk for diarrhoea and fever during the rainy season. Objective: Can changes in faecal short chain fatty acids (SCFAs) or branched chain fatty acids (BCFAs) explain the observed positive influence of probiotics and their role on nutritional status and diarrhoea...

BACKGROUND Because of changes in gut physiology, immune system reactivity, and diet, elderly people are more susceptible to gastrointestinal infections than are younger adults. The gut microflora, which provides a natural defense against invading microorganisms, changes in elderly people with the development of potentially damaging bacterial populations, which may lead to alterations in bacteri...

The probiotic industry faces the challenge of retention of probiotic culture viability as numbers of these cells within their products inevitably decrease over time. In order to retain probiotic viability levels above the therapeutic minimum over the duration of the product's shelf life, various methods have been employed, among which encapsulation has received much interest. In line with explo...

Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bif...

Measuring the viability of probiotic microorganisms in food products using plate count methodology is a common practice due to the simplicity (ease of performance), inexpensive and routine testing characters ofthis method. In present study, the suitability of de man rogosa and sharpe agar (MRS) bile agar medium forthe selective enumeration of mixed probiotic bacteria (Lactobacillus ...

Mol. Nutr. Food Res. 2023, 67, 202200385 DOI: 10.1002/mnfr.202200385 Obesity is one of the most common and chronic disease, worsening health conditions. Bifidobacterium lactis IDCC 4301 a probiotic strain previously isolated from infant feces, having anti-inflammatory properties lowering GI-symptoms. Here, anti-obesity effects have been proved by disclosing mode actions with suggestions possibl...

The objective of the present study was to evaluate the anti-inflammatory effects of Bifidobacterium animalis subsp. lactis strain BB12 in stimulated Caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. Characterization and purification studies indicate that BB12's anti-inflammatory factors might include a 50-kDa proteinaceous compound that is stable unde...

This study reports the development of several cloning vectors for bifidobacteria based on the replicon of pBC1, a cryptic plasmid from Bifidobacterium catenulatum L48 thought to replicate via the theta mode. These vectors, in which antibiotic resistance genes encoding either erythromycin or tetracycline resistance acted as selection markers, were able to replicate in a series of eight Bifidobac...

rhamnosus GG (LGG) and Bifidobacterium animalis subspecies lactis (BB12) from the Gastrointestinal (GI) Tract of Healthy Volunteers Debra D. Poutsiaka, MD, PhD, FIDSA; Lisa E. Davidson, MD; Lori Lathrop Stern, PhD, RD; Cheleste M. Thorpe, MD; Anne V. Kane, MD; Ian J. Mahoney, BA; Laura Mcdermott, BS; Rina Leyva, MS; Barry Goldin, PhD; David R. Snydman, MD, FIDSA; Division of Geographic Medicine...