Jackson, Robert W. (Purdue University, Lafayette, Ind.), and Merwin Moskowitz. Nature of a red cell sensitizing substance from streptococci. J. Bacteriol. 91:2205-2209. 1966.-A method for purifying a streptococcal antigen which sensitizes red cells to agglutination by antiserum is described. The antigen, when purified by this method, is almost exclusively composed of glycerophosphate and d-alan...

The phosphoinositide signal transduction pathway mediates important processes in intestinal physiology, yet the key enzyme, phosphoinositide-specific phospholipase C (PI-PLC), is not well-characterized in the colon. PI-PLC activity was examined in rat colonic membranes using exogenous [3H]phosphatidylinositol 4,5-bisphosphate (PIP2) as substrate, and beta-glycerophosphate to suppress degradatio...

Bone marrow cells, lymphocytes, lymph nodes, solid lymphoid tumors, spleen and ascites cells from mice implanted with leukemia have markedly decreased o-glycerophosphate and increased lactic dehydrogenase activities. A striking parallel between the ratio of these dehydrogenases and the progression of the leukemia was found. These data, in conjunction with related findings, suggest that the a-gl...

Soluble-protein and eight enzyme profiles obtained by polyacrylamide-gel electrophoresis were compared between Meloidogyne incognita and M. arenaria. Esterase, malate dehydrogenase, and alpha-glycerophosphate dehydrogenase patterns were distinctly characteristic for each species. Peroxidase and alpha-glycerophosphate dehydrogenase isoenzyme patterns varied when nematodes were propagated on diff...

1. A method is described for extraction and partial purification of mitochondrial l-3-glycerophosphate dehydrogenase from pig brain. 2. By the criteria that have so far been applied, the extraction and purification procedures do not modify the activity of the enzyme towards artificial electron acceptors. 3. The amounts of acid-liberatable flavine and iron in the preparation were measured. 4. A ...

Classification and analysis of ionic lipides and glycolipides are simplified greatly when the method of Dawson (1) for deacylation is followed by paper chromatographic separation of the resultant phosphate diesters and glycosides. With radioactive lipides the radiochromatographic method provides the sensitivity and accuracy essential for studies of the metabolism of these important compounds. T...

Rat liver microsomal dihydroxyacetone phosphate acyltransferase, in contrast to the glycerophosphate acyltransferase, was found to be active at low pH (5.5), stable towards heat (55 degrees C, 15 min) and trypsin (in the absence of detergents) and was not inhibited by high concentrations of N-ethyl maleimide. Dihydroxyacetone phosphate acyltransferase is only slightly and non-competitively inhi...

introduction: bone marrow contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. material and methods: the stromal cells of bone marrow obtained from 4 to 6 weeks old spruge-dawely male rats were grown in primary culture for 7 days and subcultured for 18 days. the cells were cultured in either dmem medium containing 15% fetal c...

Background: Sustained release delivery system can reduce the dosage frequency and maintain the therapeutic level of drugs for a longer time. Biodegradable, biocompatible and thermosensitive chitosan-beta-glycerophosphate (C-GP) solutions can solidify at body temperature and maintain their physical integrity for a longer duration. OBJECTIVES: To develop a novel delivery system based on the integ...