Benzylisoquinoline alkaloids (BIQ) are among the most structurally diverse and pharmaceutically valuable secondary metabolites. A plant-specific WRKY-type transcription factor, CjWRKY1, was isolated from Coptis japonica and identified as a transcriptional activator of BIQ biosynthesis. However, the expression of CjWRKY1 gene alone was not sufficient for the activation of genes encoding biosynth...

Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata. The use of these mass analyzers enabled tentatively identifying the alkaloids by matc...

Opium poppy (Papaver somniferum) produces a diverse array of bioactive benzylisoquinoline alkaloids and has emerged as a versatile model system to study plant alkaloid metabolism. The plant is widely cultivated as the only commercial source of the narcotic analgesics morphine and codeine. Variations in plant secondary metabolism as a result of genetic diversity are often associated with perturb...

The aim of this study was to compile a detailed phytochemical profile and assess the antioxidant properties bee-collected pollen (PBP) obtained from corn poppy (Papaver rhoeas L.) plants. To achieve this, lipid fraction prepared for quantifying fatty acids using GC-FID. Extractable alkaline-hydrolysable PBP fractions (obtained defatted sample) were used determine qualitative quantitative profil...

Simple isoquinoline alkaloids (heliamine, dehydroheliamine), a phthalide alkaloid noscapine, and an aporphine boldine are biosynthetically derived from amino acid tyrosine. These substrates simple synthetic (2-acetyl-7-amino-1,2,3,4-tetrahydroisoquinoline) contain nucleus. The biotransformation of these via reduction, oxidation, hydroxylation, N-oxidation reactions with different microorganism ...