The present study was undertaken to investigate the possibility for developing Efficient Plant regeneration protocol in Vigno mungo L. For this, the effect of Cytokinens on in vitro regeneration from Cotyledonary explants was studied in Black gram (Vigno mungo L.) Hepper Var Vamban-1. The cytokinins used were 6-benzylaminopurine (BAP), Thidiazuron (TDZ), and kinetin (Kn). The regenerated shoots...

Cotyledonary segments from the germinated immature zygotic embryos were used for somatic embryogenesis of red sandalwood (Pterocarpus santalinus). The explants established on Murashige and Skoog (MS) medium containing 5% sucrose combination 6-benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), α-Naphthaleneacetic acid. All treatments responded to callus induction with a 36–97% freq...

The present study was the first report on somatic embryogenesis of Tunisian chickpea (Cicer arietinum L.) particularly supposed to be recalcitrant and difficult manipulate in vitro. An efficient protocol has been developed for inducing indirect derived from immature zygotic embryo axis, young leaflet hypocotylar explants. Callogenesis achieved full-strength Murashige Skoog's (1962) (MS) basal m...

In vitro multiplication is an important tissue culture technique that capable of efficiently producing seedlings at any scale. It a propagation method based on the aseptic small propagules in suitable medium to enable plant regeneration. Multiplication experiments conducted set protocols adapted wild Manihot species have used modified mineral salts and MS vitamins as basic medium. Here, 25 trea...

Clonal micropropagation of plants is a modern popular method vegetative reproduction, which allows to obtain high-quality planting material in large volume short time. The research devoted the technology clonal rose Reine Sammut, particular, optimization nutrient medium composition. To increase efficiency micropropagation, silicon-containing preparation Siliplant was added composition according...

The resurrection plant Selaginella pulvinata (Hook. & Grev.) Maxim is used as an ornamental and medicinal plant. It also a good candidate for exploring the desiccation tolerance of plants. However, there not efficient propagation method S. . In present study, we evaluated establishment in vitro using frond tips explants. original shoot induction, adventitious proliferation plantlet growth m...

The tomato (Solanum lycopersicum L.) is a major crop of global economic significance. characterization genes associated with agriculturally important traits often performed using genetic transformation. To achieve an efficient transformation protocol, three components are required, namely, regenerable target tissue, DNA delivery method, and robust transformant selection system. present study wa...

The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate multiplication and that would preserve the genetic identity donor genotype (Hybrid ‘MD2’) in regenerated plants. Ten treatments, different concentrations 2,4-Dichlorophenoxyacetic (2,4-D) Picloram (P), absence presence 6-Benzylaminopurine (B...

Celastrus paniculatus, belonging to the family Celastraceae, is an important medicinal plant of India. Owing to the ever-increasing demand from the pharmaceutical industry, the species is being overexploited, thereby threatening its stock in the wild. Poor seed viability coupled with low germination restricts its propagation through sexual means. Thus, alternative approaches such as in vitro te...