The exact mechanism of apoptosis in neutrophils (PMNs) and the explanation for the antiapoptotic effect of granulocyte colony-stimulating factor (G-CSF) in PMNs are unclear. Using specific fluorescent mitochondrial staining, immunofluorescent confocal microscopy, Western blotting, and flow cytometry, this study found that PMNs possess an unexpectedly large number of mitochondria, which are invo...

Background: Rosmarinic acid (RA) is a natural phenolic compound that acts as Fyn inhibitor by 53 homology modeling of the human structure. Therefore, apoptosis mechanism related to NF-κB signaling pathway induced RA in HepG2 was investigated. Methods: The cell growth, apoptosis, and proliferation regulated various concentrations were studied. proteins expression MMP-2, MMP-9, PI3K, AKT, NF-κB, ...

Osteoarthritis (OA) is a chronic joint disease with high prevalence. However, effective treatment options for OA are still lacking. It was previously reported that LINC00473 upregulated in patients and might be associated the progression of OA; however, role remains to investigated. CHON-001 human chondrocyte cells stimulated 10 ng/mL interleukin (IL)-1β were utilized mimic vitro. Protein expre...

It is still unclear whether the BH3-only protein Puma (p53 up-regulated modulator of apoptosis) can prime cells to death and render antiapoptotic BH3-binding Bcl-2 homologues necessary for survival through its ability to directly interact with proapoptotic Bax and activate it. In this study, we provide further evidence, using cell-free assays, that the BH3 domain of Puma binds Bax at an activat...

Bromodomain and extra-terminal domain inhibitors target bromodomain-containing proteins are currently being evaluated as anti-cancer agents. In this study, we found that bromodomain extraterminal inhibitor OTX015 can effectively inhibit the proliferation of hepatocellular carcinoma cells promote apoptosis cells. At same time, anticancer effect is better than JQ1, a similar inhibitor. addition, ...

BACKGROUND AND AIMS Ghrelin is a 28-amino-acid gut hormone that was first discovered as a potent growth hormone secretagogue. Recently, it has been shown to exert a strong anti-inflammatory effect. The purpose of the study reported here was to explore the effect and mechanism of ghrelin on concanavalin (Con) A-induced acute hepatitis. METHODS Balb/C mice were divided into four groups: normal ...

BACKGROUND In this study the possible relation of Bax (an apoptosis promoter) to Bcl-2 (an apoptosis inhibitor) ratio with the apoptosis co-ordination enzyme, caspase-3, in the thymus of patients with myasthenia gravis (MG) was investigated in correlation with long-term clinical prognosis. PATIENTS AND METHODS The study included 46 patients (17M/29F, mean age 36.60 +/- 16.09 yr) with MG, who ...

Background & objective: The current study aimed to perform an immunohistochemical analysis of patterns of apoptotic and cell proliferative related protein expression in different histological grades and immune phenotypes of malignant lymphomas and other lymphoproliferative disorders Methods:This observational study was carried o...

conclusions the increasing cell toxicity, apoptosis-inducing effects and decreasing telomerase activity may play an important role in the valproic acid and radiation mechanism. the current survey suggested that it is likely beneficial to combine valproic acid and gamma radiation to treat breast cancer. results combination of valproic acid and gamma radiation increased significantly cell toxicit...