The paracrine influence of prostatic stromal cell proteins on a neoplastic prostate cell line (PA-III) was investigated. We have utilized an in vitro experimental model whereby confluent epithelial sheets of PAIII cells are grown on Matrigel-coated filters in bicameral chambers (Millicell-HA). Confluence of the epithelial sheet was confirmed mor phologically by electrical resistance measurement...

The importance of the depletion of guanine nucleotides in cellular toxicity was compared to that of the depletion of adenine nucleotides following the inhibition of early de novo purine bio synthesis in the mouse T lymphoma (S-49) cell line. Early de novo purine biosynthesis was blocked with 6-methylmercaptopurine ribonucleoside (6MMPR), while adenine and guanine nucleotide biosyntheses were in...

Parathyroid hormone-related peptide (PTHrP) was originally isolated from tumors associated with the development of hypercalcémie in vivo. Analyses of PTHrP gene expression have demonstrated that PTHrP is also produced in a wide variety of normal fetal and adult nonneoplastic tissues. The results of recent experiments have demonstrated that PTHrP is a growth factor-regulated gene, and different...

THE usually recognized technique of anaesthesia for reduction of a fractured mandible involves nasal intubation (Boyd and MacLennan, 1952), and in war injuries, which are usually of a more complicated nature, this is essential (Roche, 1945). While realizing the safety of such a technique, I feel that it still has definite disadvantages and risks, which are given below: Passage of a nasal tube. ...

The formation and removal of covalent aflatoxin B1 (AFB1): DNA adducts were studied under nontoxic conditions in meta bolically active epithelioid human lung cells (A549). Following 24 hr exposure to 0.82 @sMAFB1, the adduct level was in the range of 0.16 to 1.34 @smol adduct per mol DNA-phosphate. Approximately 60% of the total initial AFB1 adducts were removed rapidly during the first 24 hr o...

6 Many climate models are unable to capture the magnitude of observed warming and sea ice 7 decline in high northern latitudes. To understand the factors affecting the simulated warming 8 we compare the response to increasing CO2 in two pairs of climate models by decomposing 9 the 40–90◦N region heat budget. Each pair includes a member with enhanced global and 10 northern high latitude surface ...

The importance of the depletion of guanine nucleotides in cellular toxicity was compared to that of the depletion of adenine nucleotides following the inhibition of early de novo purine bio synthesis in the mouse T lymphoma (S-49) cell line. Early de novo purine biosynthesis was blocked with 6-methylmercaptopurine ribonucleoside (6MMPR), while adenine and guanine nucleotide biosyntheses were in...