Berberis tinctoria Lesch. Is an endemic plant to high hills of Nilgiris having lot of medicinal properties. For its better conservation through mass multiplication, attempts have been made to standardize tissue culture technology. The results of the study exhibited that the basal medium containing BAP and NAA each at 0.5 mg/1 was found to be the optimum for callus formation. Shoot proliferation...

Centaurium erythraea plants obtained by indirect organogenesis are described in the paper. The plants were initiated from a single adventitious shoot regenerated from callus derived from the cotyledon of a 30-day-old seedling. The shoot was multiplied on MS medium supplemented with IAA (0.1 mg·L-1) and BAP (1.0 mg·L-1). The multiplication rate (28 shoots per culture within 4 weeks) was highest ...

An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige Skoog (MS) basal medium supplemented with concentration auxins (NAA, IAA, 2,4-D) cytokinins (BAP Kn ). Different concentrations (0.5 to 4 mg/L), IAA 2mg/L), NAA 2 mg/l) cytokinin BAP mg/L) were assessed callus induction. Growth...

In vitro morphogenesis is greatly influenced by plant growth regulators (PGRs). The effect of different plant growth regulators was assessed on callus induction, in vitro shoot regeneration and hairy root formation in tomato. The maximum callus induction frequency was recorded culturing hypocotyls, while in vitro shoot regeneration frequency was significantly higher when leaf discs were used as...

Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long tender leaf base explants derived from the meristamatic region of 2-3 year old offshoots of date palm cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 and 150 mg l 2,4-dichlorophenoxy acetic acid (2,4-D) and incub...

Medium composition for the callus induction, shoot multiplication in four Indian medicinal plants, Boerhaavia diffusa, Baliospermum axillare, Abutilon indicum and Nymphoides cristatum were standardized using Murashige and Skoog`s plant tissue culture medium as the basal medium. The protocol thus standardized was further evaluated replacing sucrose and vitamins with the fruit pulp of Borassus fl...

A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bas...

OBJECTIVES This study compared callus formation in distal femur fractures stabilized with locking plates and intramedullary nails to test the hypothesis that locking plates induce less fracture callus than IM nails. DESIGN Retrospective case matched study. SETTING Two orthopaedic trauma centers. PATIENTS 174 distal femur fracture were reviewed to extract cases treated with retrograde IM n...

Wild pistachio (Pistacia atlantica subsp. kurdica) is one of tree species that cover much of the forest in northwest of Iran and used in industries, medicine and as food. The present study describes callus induction of the P. atlantica subsp. kurdica using seedling stem explants and the influence of different plant growth regulators including kinetin, benzyladenine (BA), thidiazuron (TDZ), 6Ben...