results in total, 80 food samples suspected of seb contamination were assessed using the two methods. fifty-four samples were contaminated based on the pcr technique and twenty-six of those were confirmed using the strip assay. conclusions the sensitivity of the sandwich method was approximately 10 ng/ml and that of the competitive method was approximately 250 ng/ml. in the lfd, a highly specif...

The validity of measuring vaginal sialidase activity to identify bacterial vaginosis (BV) was determined by using 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid in a near-patient test. The sensitivity and specificity of the test for prediction of BV were 95.6 and 96.3%, respectively. Positive and negative predictive values were 95.6 and 96.3%, respectively. This test may be an alter...

OBJECTIVE To evaluate sensitivities at the herd level of test strategies used in the Voluntary Johne's Disease Herd Status Program (VJDHSP) and alternative test strategies for detecting dairy cattle herds infected with Mycobacterium paratuberculosis. DESIGN Nonrandom cross-sectional study. SAMPLE POPULATION 64 dairy herds from Pennsylvania, Minnesota, Colorado, Ohio, and Wisconsin. Fifty-si...

introduction: escherichia coli (e.coli) as a main cause of both nosocomial and community-acquired infections in humans have a relative potential to develop resistance. nowadays, most infections caused by esbl-producing e.coli (esblec) had mostly been described as nosocomial acquired or nursing home related. in this study, we employed e-test assay to detect antibiotic resistance of e.coli strain...

Introduction Bacterial meningitis is a known infectious disease which occurs at early ages and should be promptly diagnosed and treated. Bacterial and aseptic meningitis are hard to be distinguished. Therefore, physicians should be highly informed and experienced in this area. The main aim of this study was to suggest a system for distinguishing between bacterial and aseptic meningitis, using f...

Chemoreceptors in Escherichia coli are coupled to the flagella by a labile phosphorylated intermediate, CheY approximately P. Its activity can be inferred from the rotational bias of flagellar motors, but motor response is stochastic and limited to a narrow physiological range. Here we use fluorescence resonance energy transfer to monitor interactions of CheY approximately P with its phosphatas...

fire blight, a bacterial disease caused by erwinia amylovora (burr.) winslow et al. 1920 is considered as a serious disease on pome fruits. during recent years, this disease has caused serious damages to the fruit growing industry in iran. precise and reliable methods for identification and detection of the pathogen can help in its early control, prevent the disease spread and help eliminate th...

background & aim: by increasing resistance of fish pathogens to chemical drugs, using herbal plants with antimicrobial properties is necessary.in this study, the composition of the essential oil and antimicrobial activity of aerial parts of achillea wilhelmsii c.koch growing wild in zagross mountain (southwest of iran) was analyzed against common bacterial and fungal pathogens of fish. expe...

Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococc...