Optimization of the Culture Medium, Fermentation Process, and Effectiveness a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2)

Which genes have been incorporated into the Marucaresistant cowpea? The Maruca-resistant cowpea contains the cry1Ab (Bt) and nptII genes. The Bt gene was derived from the soil bacterium Bacillus thuringiensis subsp. kurstaki. The Bt protein produced in modified cowpea plants has been shown to be identical to the naturally occurring protein, and equivalent to the protein used as a biopesticide b...

53 The microbial insecticide, Bacillus thuringiensis (Bt), has become the mainstay of non-chemical control of 54 Lepidopteran pests, either as sprays or through the incorporation of Bt toxins into transgenic crops. Given 55 the wide use of Bt, it is striking that currently only one pest species, Plutella xylostella, has been reported 56 to have developed significant resistance to Bt outside the...

Bacillus thuringiensis var. thuringiensis strain T01-328, isolated from Cubatão county (São Paulo State, Brazil), produces a soluble pesticide protein, Cry1Ia, during vegetative growth. Here, we report the 7.089-Mbp draft genome sequence, composed of a 5.5-Mb chromosome and 14 plasmids, which is the largest B. thuringiensis genome sequenced to date.

A 5.8-kb fragment of the large conjugative plasmid pAW63 from Bacillus thuringiensis subsp. kurstaki HD73 containing all the information for autonomous replication was cloned and sequenced. By deletion analysis, the pAW63 replicon was reduced to a 4.1-kb fragment harboring four open reading frames (ORFs). Rep63A (513 amino acids [aa]), encoded by the largest ORF, displayed strong similarity (40...

Four sunflower accessions were compared with a susceptible check, hybrid '894', in the greenhouse to determine their resistance to the banded sunflower moth, Cochylis hospes Walsingham, and their interaction with Bacillus thuringiensis Berliner variety kurstaki. Antibiosis, expressed as lower larval weight, was detected in all of the accessions. In addition to being antibiotic, sunflower access...

Populations of diamondback moth, Plutella xylostella (L.), sampled from commercial fields of crucifers in three states of Mexico, were tested for susceptibility to commercial formulations of Bacillus thuringiensis subsp. kurstaki (Berliner) (Dipel 2X), B. thuringiensis subsp. aizawai (XenTari), delta endotoxin Cry 1C (MC), and CryIA(c) (MVP), and a mixture of B. thuringiensis subsp. kurstaki an...

The cry1Ac gene of Bacillus thuringiensis subsp. kurstaki HD-73 (B. thuringiensis HD-73) is a typical example of a sporulation-dependent crystal gene and is controlled by sigma E and sigma K during sporulation. To monitor the production and accumulation of Cry1Ac at the cellular level, we developed a green fluorescent protein-based reporter system. The production of Cry1Ac was monitored in spo0...

Tomato is one of the most popular vegetables grown in South Asian countries, including India. Introduction any invasive species to a new environment has always resulted serious ecological and economical threats globally. One such widely effected pest tomato cultivation Tuta absoluta, which was reported infesting fields India 2014 for first time. In order reduce over reliance on chemical pestici...

Under glasshouse conditions, various cornstarches and adjuvants were examined as encapsulating agents in sprayable formulations for Bacillus thuringiensis subsp. kurstaki Berliner. When these materials were suspended in water and tested for physical factors such as viscosity, clumping, and retention on plant surfaces, a range of responses was observed. All formulations tested had acceptable vis...