Bacillus coagulans P38 is an efficient polymer-grade l-lactic acid producer from a cellulosic carbon source. Here, the draft 3.37-Mb genome sequence of this potential strain may provide useful information to further improve the strain performance for higher titers and, importantly, to understand the mechanism of its high tolerance for 2-furfural.

An in vivo experiment was conducted to study the effects of probiotic Bacillus coagulans spores, with and without prebiotic, inulin, on gastrointestinal (GI) microbiota of healthy rats and its potentiality to survive in the GI tract. Forty-eight male Wistar rats were randomly divided into four groups (n=12) and fed as follows: standard diet (control), standard diet supplied with 5% w/w long cha...

A green house nursery study was conducted to assess the interaction between arbuscular mycorrhizal (AM) fungus, Glomus aggregatum and some plant growth promoting rhizomicrooganisms (PGPR's), Bacillus coagulans and Trichoderma harzianum, in soil and their consequent effect on growth, nutrition and content of secondary metabolities of Solanum viarum seedlings. Triple inoculation of G. aggregatum+...

Several spore-forming strains of Bacillus are marketed as probiotics due to their ability to survive harsh gastrointestinal conditions and confer health benefits to the host. We report the complete genomes of two commercially available probiotics, Bacillus coagulans S-lac and Bacillus subtilis TO-A JPC, and compare them with the genomes of other Bacillus and Lactobacillus. The taxonomic positio...

AIMS To characterize the antimicrobial protein produced by Bacillus coagulans used in the probiotic dietary supplement (Lactospore) Probiotic, Sabinsa Corp., Piscataway, NJ, USA). METHODS AND RESULTS Bacillus coagulans ATCC 7050 was grown at 37 degrees C for 18 h. The cell free supernatant was concentrated 10-fold (lactosporin preparation, LP). The antimicrobial activity of LP was confirmed a...

The protonophore-mediated collapse of the large delta pH that acidophiles maintain across their cytoplasmic membranes was augmented by the presence of Cl-, and Cl- influx into the cells occurred evidently in response to the protonophore-induced increase in the inside-positive membrane potential (+ delta psi). In respiring cells, the addition of Cl- but not SO4(2-) salts caused a rapid and preci...

Probiotics are microorganisms which when administered in adequate amounts confer health benefits to the host. A leading pharmaceutical company producing Bacillus coagulans as a probiotic was facing the problem of recurring phage attacks. Two mutants viz. B. co PIII and B. co MIII that were isolated as phage resistant mutants after UV irradiation and MMS treatment of phage sensitive B. coagulans...

Effects of high temperature pretreatment and inoculation Bacillus coagulans were determined relative to the physicochemical properties bacterial community aerobic composting chicken manure. Chicken manure was pretreated with for 0 h (CJ), 0.5 (T-0.5h), 1.0 (T-1.0h), 1.5 (T-1.5h), 2.0 (T-2.0h) then inoculated B. coagulans. without included as control (CK). The results showed that in CJ, T-0.5h, ...

The experiment was carried out to examine the organic acids production of rice straw fermented with some types of microorganisms at different temperatures. The experiment was designed as Split Plot-Completely Randomized Design. The main plot was temperatures treatments (25, 35, 45°C) and the sub plot were microorganisms (Control, Control+Mollases, Lactobacillus fermentum, Bacillus subtilis, Bac...

Bacillus coagulans 2-6 is an excellent producer of optically pure l-lactic acid. However, little is known about the mechanism of synthesis of the highly optically pure l-lactic acid produced by this strain. Three enzymes responsible for lactic acid production-NAD-dependent l-lactate dehydrogenase (l-nLDH; encoded by ldhL), NAD-dependent d-lactate dehydrogenase (d-nLDH; encoded by ldhD), and gly...