By using membrane preparations from B. licheniformis, this enzyme has been assayed in both directions and found to be resistant to tunicamycin (1OOpg. ml-'). Therefore in wall-polymer assembly in B. licheniformis, B . subtilis and S . aureus tunicamycin has been shown to inhibit the biosynthesis of both peptidoglycan and the linkage units involved in the attachment of teichoic acids to peptidog...

Tryptophan, phenylalanine, tyrosine, and several other metabolites are all synthesized from a common precursor, chorismic acid. Since tryptophan is a product of an energetically expensive biosynthetic pathway, bacteria have developed sensing mechanisms to downregulate synthesis of the enzymes of tryptophan formation when synthesis of the amino acid is not needed. In Bacillus subtilis and some o...

Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright. Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis. J. Bacteriol. 88:220-225. 1964.-Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected fi...

Purpose: Robust pharmaceutical and industrial enzymes from extremophile microorganisms are main source of enzymes with tremendous stability under harsh conditions which make them potential tools for commercial and biotechnological applications. Methods: The genome of a Gram-positive halo-thermotolerant Bacillus sp. SL1, new isolate from Saline Lake, was investigated for the presence of genes co...

Four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. The four strains, 24K(T), 28K(T), 41KF2a(T) and 41KF2b(T), were identified as members of the genus Bacillus. Phylogenetic analysis based on 16S rRNA gene sequences indicated that three of the strains, 24K(T), 28K(T) and 41KF2a(T), are very similar to one another (>98 % s...

BACKGROUND Selection of an appropriate host organism is crucial for the economic success of biotechnological processes. A generally important selection criterion is a low maintenance energy metabolism to reduce non-productive consumption of substrate. We here investigated, whether various bacilli that are closely related to Bacillus subtilis are potential riboflavin production hosts with low ma...

The synthesis of teichoic acids has been examined during germination in Bacillus licheniformis ATCC 9945 and in B. subtilis W-23. Teichoic acids are absent from the spores of both organisms. B. licheniformis spores lack the enzymes responsible for teichoic acid synthesis. The appearance of these enzymes during germination is correlated with the appearance of teichoic acids in the cell. The appe...

The neomycin phosphotransferase gene (neo) from Transposon Tn5 is active in Gram-negative bacteria but silent in B. subtilis since it lacks an appropriate ribosome binding site for Gram-positive bacteria. Neo translation could be reactivated by coupling its initiation to the translational termination of the highly expressed beta-lactamase gene (penP) from B. licheniformis. This initiation occur...

This study was carried out to evaluate the effect of probiotic (Bacillus latrospores and Bacillus licheniformis) on growth and survival of Silver carp larvae (Hypophthalmichthys molitrix). Rotifers (Brachionus plicatilis) were used as live prey vehicle for probiotic transport. They were cultured in sea water with 15 ppt salinity and Nannochloropsis oculata microalgae was used as feed. Five trea...

The structural gene for alkaline phosphatase (orthophosphoric monoester phosphohydrolase; EC 3.1.3.1) of Bacillus licheniformis MC14 was cloned into the Pst1 site of pMK2004 from chromosomal DNA. The gene was cloned on an 8.5-kilobase DNA fragment. A restriction map was developed, and the gene was subcloned on a 4.2-kilobase DNA fragment. The minimum coding region of the gene was localized to a...