Synthetic microporous membranes with functional groups covalently attached were used to selectively separate beta-lactoglobulin, BSA, and alpha-lactalbumin from rennet whey. The selectivity and membrane performance of strong (quaternary ammonium) and weak (diethylamine) ion-exchange membranes were studied using breakthrough curves, measurement of binding capacity, and protein composition of the...

The fluorescence yield of bound retinol in human retinolbinding protein (RBP) and irs prealbumin complex (PARBP) were found to be nearly the same and to exceed that of free retinol in petroleum ether by about 9-fold. Bovine serum albumin and P-lactoglobulin also formed strongly fluorescent water-soluble complexes with retinol. The fluorescence yield of retinol complexed with bovine serum albumi...

In 1934 Palmer (l), in R. K. Cannan’s laboratory, succeeded in obtaining from the plasma of cow’s milk a crystalline protein with the general properties of a globulin, which was subsequently designated as /3-lactoglobulin (2). According to Palmer (1) and to Sorensen and Sgrensen (3) from 50 to 60 per cent of the whey proteins can bc obtained in the form of this globulin. Detailed physicochemica...

Abstract The most allergenic protein in cow’s milk for new-borns is Beta Lactoglobulin (β-LG), which also one of the bovine whey proteins. As a result, choosing suitable animals to create free essential. In this study, Indonesian Friesian Holstein’s genetic variants β-LG gene were examined). Forty-two blood samples sampled as DNA sources. allele and genotype variant identified using PCR-RFLP, o...

Background Previous animal studies showed that partial whey hydrolyates could prevent acute allergic symptoms in a mouse model for cow’s milk allergy. This effect was even more pronounced in combination with a diet containing a specific non-digestible oligosaccharide mixture of scGalacto(scGOS), lc-Fructo(lcFOS) and Acidic(pAOS) oligosaccharides (in a 9:1:2 ratio). We hypothesized that these hy...

Methods b-Lactoglobulin, whole casein and its four different fractions, i.e. as1-, as2-, band -caseins, were isolated from raw CM and GM. Purified b-caseins were subjected to a mild proteolysis by plasmin which generated 3 peptides, i.e. f(1-28), f(29-107) and f(108-207/9). Synthetic peptides partially recovering the N-terminal f(29107) part of the caprine b-casein were also produced. Immunorea...