We present a paternity testing case in which a double incompatibility was found for two short tandem repeat (STR) markers, human fibrinogen alpha (FGA) and Penta E. Analysis of the trio (mother, father, and daughter) included the amplification with a battery of 15 autosomal short tandem repeats (STR) by using a commercially available PowerPlex 16 System kit, and the detection with an ultraviole...

Analyzing microsatellite instability (MI) in malignant tumors is thought to be useful for screening cancer patients to identify those patients with a higher risk of developing second malignant tumors. In this paper, we report a new, accurate, and efficient method of detecting MI using an automated fluorescent DNA sequencer and a computer that automatically calculates the size, height, and area ...

The Amoeba group communication system has two unique aspects: (1) it uses a sequencer-based protocol with negative acknowledgements for achieving a total order on all group messages; and (2) users choose the degree of fault tolerance they desire. This paper reports on our design decisions in retrospect, the performance of the Amoeba group system, and our experiences using the system. We conclud...

Differential display is a method for the survey, analysis and comparison of gene expression in eukaryotic cells and tissues. Differential display involves isolation of high-quality nondegraded RNA, selective reverse transcription of polyadenylated mRNA using specific anchored oligopolydeoxythymidine [oligo(dT)] primers, and the subsequent PCR amplification of the cDNA with the same oligo(dT), a...

Reductions in operations personnel and the availability of more capable automation technology have resulted in increased automation of space control systems. One of the challenges of deploying autonomous systems is ensuring that people can interact effectively with them. An important strategy for such interaction is providing for different levels of control autonomy, or adjustable autonomy. At ...

Elimination of the data processing bottleneck in high-throughput sequencing will require both improved accuracy of data processing software and reliable measures of that accuracy. We have developed and implemented in our base-calling program phred the ability to estimate a probability of error for each base-call, as a function of certain parameters computed from the trace data. These error prob...

In this paper we present the specification of a sequencer service that allows independent processes to get a sequence number that can be used to label successive operations (e.g. to allow a set of independent and concurrent processes to get a total order on their operations). Moreover, we provide an implementation of the sequencer service in a specific partially synchronous distributed system, ...

vectors may also be affected depending on whether these regions contribute to this activity (14). Interestingly , although pGL3-basic (Promega, Madison, WI, USA) is derived from pUC vectors, it lacks many of the AP1 motifs found in pBLCAT3; however, it is still transactivated in experiments where other transcription factors such as NF-Y, c-Ets-2, PEA3, Sp1, or GATA-4 are overexpressed (D.A. You...

The availability of massive amounts of DNA sequence information has begun to revolutionize the practice of biology. As a result, current large-scale sequencing output, while impressive, is not adequate to keep pace with growing demand and, in particular, is far short of what will be required to obtain the 3-billion-base human genome sequence by the target date of 2005. To reach this goal, impro...