Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to ...

The Aspergillus nidulans flbD gene encodes a protein with a Myb-like DNA-binding domain that is proposed to act in concert with other developmental regulators to control initiation of conidiophore development. We have identified a Neurospora crassa gene called rca-1 (regulator of conidiation in Aspergillus) based on its sequence similarity to flbD. We found that N. crassa rca-1 can complement t...

Fungal infections of the maxillary sinus are frequently caused by Aspergillus species, particularly A. fumigatus. In otherwise healthy persons there is an association with overfilling of dental root canals, when zinc-containing filling materials were used. Below, a maxillary sinus aspergilloma is reported in a young immunocompetent female patient caused by Aspergillus (Emericella) nidulans.

Aspergillus nidulans is an important experimental organism, and it is a model organism for the genus Aspergillus that includes serious pathogens as well as commercially important organisms. Gene targeting by homologous recombination during transformation is possible in A. nidulans, but the frequency of correct gene targeting is variable and often low. We have identified the A. nidulans homolog ...

Susceptibility testing of 27 Aspergillus reference strains belonging to five species was performed using the microdilution broth method with yeast nitrogen broth and RPMI-1640. Similar results were found using the two media. The strains of Aspergillus fumigatus (n = 8) and Aspergillus niger (n = 4) had MICs of amphotericin B in the range 0.125-0.5 mg/L. In contrast, nine out of 13 strains of As...

The present study deals with the phylogenetic analysis of A. nidulans group and assay of how much A. aegyptiacus belongs to A. nidulans group or A. versicolor group. The revealed results can be summarized as follows: 1The eight fungal isolates used in this investigation were identified as two isolates of Aspergillus aegyptiacus (AUMC 6122 & 3603),E. nidulans (AUMC152), E. rugulosa (AUMC164), E....