A rapid, simple and stable biosensor for aspartame detection was developed. Alcohol oxidase (AOX), carboxyl esterase (CaE) and bovine serum albumin (BSA) were immobilised with glutaraldehyde (GA) onto screen-printed electrodes modified with cobalt-phthalocyanine (CoPC). The biosensor response was fast. The sample throughput using a flow injection analysis (FIA) system was 40 h⁻¹ with an RSD of ...

The effect of aspartame on circadian rhythms of calcium and inorganic phosphorus levels was studied in rats. Acrophase delays in calcium rhythms and advances in inorganic phosphorus rhythms and alteration in mesor values in both rhythms were observed in aspartame-treated rats. However, no change in amplitude values was observed. Oral administration of aspartame leads to increased levels of aspa...

This study reviews clinical studies testing the effects of various doses of aspartame on blood levels of phenylalanine, aspartate, and methanol in normal subjects and known phenylketonuric heterozygotes. The effect of aspartame on the phenylalanine-to-large neutral amino acid ratio under various feeding situations is shown. The clinical studies of aspartame in diabetic subjects are limited to o...

The fairly widespread use of the artificial sweetener aspartame (N-L-a-aspartyl-L phenylalanine 1-methyl ester), marketed under brand names such as Canderel, Equal, NutraSweet, and Tri-Sweet, has engendered considerable controversy, inchuding suggestions of significant neu rochemical changes.' Data presented by Wurtman' indicate that aspartame alone can almost double rat brain pbenylalanine lev...

Aspartame is a dipeptide (L-aspartyl-L-phenylalanyl methyl ester) with a sweetening potential of 180 to 200 times that of sucrose. Questions have been raised about the potential toxic effects of its constitu ent amino acids when the compound is ingested in large amounts by lactating women. Plasma, erythrocyte and milk levels of free amino acids were measured in six normal female subjects with e...

The aim of this study was to investigate the effect of citric acid or the sweetening agent aspartame on the CCl4-induced hepatic injury in rats. Citric acid (10 mg/kg, 100 mg/kg or 1000 mg/kg), aspartame (0.625 or 1.25 mg/kg) or silymarin (25 mg/kg) was given once daily orally simultaneously with CCl4 and for one week thereafter. The administration of citric acid at 100 mg/kg or 1000 mg/kg to C...

Aspartame is a methyl ester of a dipeptide of aspartic acid and phenylalanine. It is 200× sweeter than sucrose and is approved for use in food products in more than 90 countries around the world. Aspartame has been evaluated for genotoxic effects in microbial, cell culture and animal models, and has been subjected to a number of carcinogenicity studies. The in vitro and in vivo genotoxicity dat...

In a study of the impact of aspartame, fat, and carbohydrate on appetite, we monitored blood glucose continuously for 431 (SE 16) min. Ten healthy males (19-31 years) participated in three time-blinded visits. As blood glucose was monitored, appetite ratings were scored at randomized times. On the first meal initiation, volunteers consumed one of three isovolumetric drinks (aspartame, 1 MJ simp...

Aspartame (APM), a newly available synthetic sweetener approximately 180-200 times sweeter than sucrose, was approved for marketing by the FDA in 1981 following several years of scientific review. It is available in the United States for use as a sweetener in breakfast cereals, powdered beverages, gelatins, puddings, fillings, whipped toppings, chewing gum, soft drinks and as a table-top sweete...