Simultaneous administration of 6-chloropurine and azaserine in the therapy of mice bearing the ascites cell forms of Sarcoma 180 or the Ehrlich carcinoma resulted in a potentiation of tumor inhibition as measured by increases in survival time. The 6C3HED and the Mecca ascites lymphosarcomas proved resistant to the combination of chloropurine and azaserine employed. Hepatoma 134 was sensitive to...

The inhibitory effects of protease inhibitors on blood-borne metastasis in male Donryu rat lung were studied. Injection i.v. of 10(6) Yoshida ascites hepatoma AH7974 cells induced about 118 +/- 92 (S.D.) metastatic foci in rat lung after 3 weeks. Leupeptin (50 mg/kg body weight twice a day), injected i.p. from 2 days before to 4 days after the inoculation of tumor cells, reduced the number of m...

Cyclosporin A abrogates pleiotropic drug resistance in certain experimental tumors. Its impact on drug-sensitive tumors has not been investigated. Our studies show that in drug-sensitive Ehrlich ascites carcinoma and hepatoma 129 cyclosporin A enhances daunorubicin inhibition of DNA synthesis in vitro and prolongs survival of host mice in vivo. Of particular interest is that cyclosporin A conve...

The activity of the malate-aspartate shuttle for the reoxidation of cytoplasmic reduced nicotinamide adenine dinucleotide (NADH) by mitochondria was assessed in six lines of rodent ascites tumor cells (two strains of Ehrlich ascites carcinoma, Krebs II carcinoma, Novikoff hepatoma, AS-30D hepatoma, and L1210 mouse leukemia). All the tumor cells examined showed mitochondrial reoxidation of cytop...

Further detailed observation has been made on the ultrastructure of the six strains of Yoshida ascites hepatoma representing various growth rates and pathological characteristics. Common to all the ascites hepatoma were the large, irregular nuclei with hypertrophied nucleoli, increased free ribosomes, and no microbodies. The present investigation throughout many transplant generations has revea...

Our previous studies have shown that a cell surface-associated adhesive factor (AF), separated from rat ascites hepatoma AH136B cells of a differentiated type and highly purified by chromatography, induces not only aggregation of dissociated AH136B cells or rat ascites hepatoma AH109A cells of an undifferentiated type but also adhesiveness characterized by the development of junctional complexe...