Background and aims: Since accurate forecasts help inform decisions for preventive health-careintervention and epidemic control, this goal can only be achieved by making use of appropriatetechniques and methodologies. As much as forecast precision is important, methods and modelselection procedures are critical to forecast precision. This study aimed at providing an overview o...

Abstract A transformation network describes how one set of resources can be transformed into another via technological processes. Transformation networks in economics are useful because they can highlight areas for future innovations, both in terms of new products, new production techniques, or better efficiency. They also make it easy to detect areas where an economy might be fragile. In this ...

A coupled air–sea general circulation model is used to simulate the global circulation. Different parameterizations of lateral mixing in the ocean by eddies, horizontal, isopycnal, and isopycnal plus eddy advective flux, are compared from the perspective of water mass transformation in the Southern Ocean. The different mixing physics imply different buoyancy equilibria in the surface mixed laye...

Introduction and expression of multiple transgenes is frequently required for basic and applied studies. However, at present, multigene transformation is very difficult due to technical limitations of existing methods. Here, we describe a vector system for efficient multigene assembly and transformation. The system consists of a transformation-competent artificial chromosome (TAC)-based accepto...

In order to determine the mechanisms involved in the persistence of extracellular DNA in soils and to monitor whether bacterial transformation could occur in such an environment, we developed artificial models composed of plasmid DNA adsorbed on clay particles. We determined that clay-bound DNA submitted to an increasing range of nuclease concentrations was physically protected. The protection ...

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...