1 During the last years, the use of type III secretion system-based bacterial vectors for 2 immunotherapy purpose has been assessed in various applications. We showed that a type 3 III secretion-based P. aeruginosa vector delivering the OVA antigen induced an efficient 4 specific CD8+ T lymphocyte immune response against OVA-expressing cells. Because of 5 the intrinsic toxicity of the vector, f...

Salmonella enterica serovar Typhimurium is a versatile host organism for the generation of recombinant live vaccines for mucosal immunization. We investigated the performance of an intracellular-activated promoter for expression of heterologous antigens by Salmonella carrier strains. We observed that intracellular expressed antigens were more efficient in elicitation of humoral and cellular imm...

Pasteurella multocida serotype B:2 is the etiological agent of haemorrhagic septicaemia (HS) in cattle and buffaloes, a disease that causes significant economic losses to the dairy industry in India. Several killed vaccines are in uses that provide protection to limited period of time. The aim of this study was to generate an attenuated strain of Pasteurella multocida P52 by targeted gene knock...

The gene (aroA) of Actinobacillus pleuropneumoniae, serotype 2, encoding 5-enolpyruvylshikimate-3-phosphate synthase was cloned by complementation of the aroA mutation in Escherichia coli K-12 strain AB2829, and the nucleotide sequence was determined. A pair of primers from the 5' and 3' termini were selected to be the basis for development of a specific PCR assay. A DNA fragment of 1,025 bp wa...

During the last few years, the use of type III secretion system-based bacterial vectors for immunotherapy purposes has been assessed in various applications. We showed that a type III secretion-based Pseudomonas aeruginosa vector delivering the ovalbumin (OVA) antigen induced an efficient specific CD8 ؉ T-lymphocyte immune response against OVA-expressing cells. Because of the intrinsic toxicity...

The chemolithoautotroph NT-26 oxidizes arsenite to arsenate by using a periplasmic arsenite oxidase. Purification and preliminary characterization of the enzyme revealed that it (i) contains two heterologous subunits, AroA (98 kDa) and AroB (14 kDa); (ii) has a native molecular mass of 219 kDa, suggesting an alpha2beta2 configuration; and (iii) contains two molybdenum and 9 or 10 iron atoms per...

Salmonellae have evolved several mechanisms to evade host clearance. Here, we describe the influence on bacterial immune escape of the effector protein SopB, which is translocated into the cytosol through a type III secretion system. Wild-type bacteria, as well as the sseC and aroA attenuated mutants exerted a stronger cytotoxic effect on dendritic cells (DC) than their SopB-deficient derivativ...